Projecten per jaar
Samenvatting
Analytical techniques hyphenated with mass-spectrometry, such as high-performance liquid chromatography – mass spectrometry (HPLC–MS), have become indispensable tools for the analysis of complex protein samples. However, to reduce spectral complexity, and to minimize ion suppression of low abundant species, a high-performance LC separation is required prior to MS analysis. A major bottleneck is the limited separation power of the LC strategies currently used. For fast separations with a total analysis time of 10 min, the peak capacity that can be reached with column-based one- and two-dimensional LC systems approaches 200 and 600, respectively, while higher peak capacities come at the expense of long analysis times. Alternatively, for so-called spatial three-dimensional (3D)-LC components are separated in the space domain with each peak being characterized by its coordinates in a three-dimensional separation body. Spatial 3D-LC has the potential to offer unprecedented resolving power, since the total peak capacity is the product of the three individual peak capacities. Due to parallel developments during the second- and third-dimension separations, the analysis time is greatly reduced compared to a coupled-column multi-dimensional LC approach. Our research addresses the different design aspects to create a microfluidic chip for spatial 3D-LC. The proposed microfluidic device featuring 16 parallel second-dimension channels and 256 parallel third-dimension channels can potentially yield a peak capacity of 8000 in a total analysis time of 10 minutes.
Originele taal-2 | English |
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Titel | Oral presentation at the 14th International Symposium on Hyphenated Techniques in Chromatography and Separation Technology (HTC-14), January 25-29, 2016, Gent, Belgium |
Uitgeverij | LDO |
Pagina's | 125-125 |
Status | Published - 25 jan 2016 |
Evenement | 14th International Symposium on Hyphenated Techniques in Chromatography and Separation Technology (HTC-14) - Ghent, Belgium Duur: 27 jan 2016 → 29 jan 2016 |
Conference
Conference | 14th International Symposium on Hyphenated Techniques in Chromatography and Separation Technology (HTC-14) |
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Land/Regio | Belgium |
Stad | Ghent |
Periode | 27/01/16 → 29/01/16 |
Vingerafdruk
Duik in de onderzoeksthema's van 'Design Aspects for the Construction of a Microfluidic Device for Comprehensive Spatial Three-Dimensional Liquid Chromatography'. Samen vormen ze een unieke vingerafdruk.Projecten
- 1 Afgelopen
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SRP6: SRP (Zwaartepunt): exploitatie van de voordelen van de Orde in Opsluiting voor een groenere chemie
Desmet, G., Denayer, J., Denayer, J., Desmet, G. & Denayer, J.
1/11/12 → 31/10/22
Project: Fundamenteel