Design Aspects for the Construction of a Microfluidic Device for Comprehensive Spatial Three-Dimensional Liquid Chromatography

Bert Wouters, Ekaterina Davydova, Sam Wouters, Gabriel Vivo Truyols, Peter Schoenmakers, Sebastiaan Eeltink

Onderzoeksoutput: Meeting abstract (Book)

Samenvatting

Analytical techniques hyphenated with mass-spectrometry, such as high-performance liquid chromatography – mass spectrometry (HPLC–MS), have become indispensable tools for the analysis of complex protein samples. However, to reduce spectral complexity, and to minimize ion suppression of low abundant species, a high-performance LC separation is required prior to MS analysis. A major bottleneck is the limited separation power of the LC strategies currently used. For fast separations with a total analysis time of 10 min, the peak capacity that can be reached with column-based one- and two-dimensional LC systems approaches 200 and 600, respectively, while higher peak capacities come at the expense of long analysis times. Alternatively, for so-called spatial three-dimensional (3D)-LC components are separated in the space domain with each peak being characterized by its coordinates in a three-dimensional separation body. Spatial 3D-LC has the potential to offer unprecedented resolving power, since the total peak capacity is the product of the three individual peak capacities. Due to parallel developments during the second- and third-dimension separations, the analysis time is greatly reduced compared to a coupled-column multi-dimensional LC approach. Our research addresses the different design aspects to create a microfluidic chip for spatial 3D-LC. The proposed microfluidic device featuring 16 parallel second-dimension channels and 256 parallel third-dimension channels can potentially yield a peak capacity of 8000 in a total analysis time of 10 minutes.
Originele taal-2English
TitelOral presentation at the 14th International Symposium on Hyphenated Techniques in Chromatography and Separation Technology (HTC-14), January 25-29, 2016, Gent, Belgium
UitgeverijLDO
Pagina's125-125
StatusPublished - 25 jan 2016
Evenement14th International Symposium on Hyphenated Techniques in Chromatography and Separation Technology (HTC-14) - Ghent, Belgium
Duur: 27 jan 201629 jan 2016

Conference

Conference14th International Symposium on Hyphenated Techniques in Chromatography and Separation Technology (HTC-14)
Land/RegioBelgium
StadGhent
Periode27/01/1629/01/16

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