Detection and Analysis of SUMOylation Substrates In Vitro and In Vivo

Cesyen Cedeño, Esther La Monaca, Mara Esposito, Gustavo Gutierrez Gonzalez

Onderzoeksoutput: Articlepeer review

Samenvatting

SUMOylation is a widely used protein posttranslational mechanism capable of regulating substrates localization, stability, and/or activity. Identification and characterization of bona fide SUMO substrates is a laborious task but its discovery can shed light to exquisite and crucial regulatory signaling events occurring within the cell. Experiments performed in the SUMOylation field often demand a good understanding of the putative substrate's function and necessitate a solid knowledge regarding both in vitro and in vivo approaches. This contribution offers a simplified view into some of the most common experiments performed in biochemical and cell biological research of the SUMO pathway in mammalian systems. It also summarizes and updates well established protocols and tricks in order to improve the likelihood to obtain reliable and reproducible results.

Originele taal-2English
Pagina's (van-tot)267-278
Aantal pagina's12
TijdschriftMethods in Molecular Biology
Volume1449
DOI's
StatusPublished - 2016

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