Development of human liver cancer 3D spheroid cultures for assessment of nanoplastic toxicity

Sybren De Boever, Kaat Leroy, Lindsey Devisscher, Mathieu Vinken

Onderzoeksoutput: Poster

Samenvatting

3D spheroid cell cultures are an appropriate alternative to conventional 2D monolayer cell systems because of a higher in vivo similarity. However, their use is currently underrepresented in hazard identification, in casu in the field of particle toxicity. In the present study, we focus on the development of 3D spheroid cultures of 7 human liver cancer cell lines and test their interaction with micro- and nanoplastics. Data regarding spheroid growth and viability indicated that 3 out of the 7 cell lines, namely C3A, SK-HEP-1 and Hep3B cells, formed maintainable 3D spheroids over time in non-scaffold culture conditions. A superior sensitivity towards the hepatotoxic compounds menadione and carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone was demonstrated for the spheroid-forming cell lines compared to their 2D counterparts. Fluorescent polystyrene particles with a diameter of 0.5 µm interacted with the 3D spheroid cultures in a dose-, time- and cell type-dependent manner. However, larger particles with a diameter of 5 µm were incompatible with the scaffold-free set-up because of excessive particle precipitation, thereby preventing particle-spheroid interaction. The successful development of maintainable spheroid cultures and their potential to interact with nanosized particles advocates their use in the area of particle toxicity.
Originele taal-2English
StatusPublished - 19 sep 2023
EvenementJoint 3R Symposium - Emerging fields in 3Rs - Brussels, Belgium
Duur: 19 sep 202321 sep 2023

Conference

ConferenceJoint 3R Symposium - Emerging fields in 3Rs
Land/RegioBelgium
StadBrussels
Periode19/09/2321/09/23

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