Direct detection and identification of African trypanosomes by fluorescence in situ hybridization with peptide nucleic acid probes

M Radwanska, S Magez, H Perry-O'Keefe, H Stender, J Coull, J M Sternberg, P Büscher, J J Hyldig-Nielsen

Onderzoeksoutput: Article

31 Citaten (Scopus)

Samenvatting

We have developed a rapid and easy to perform fluorescence in situ hybridization test that allows specific identification of trypanosomes from the subgenus Trypanozoon, using peptide nucleic acid probes. Probes were designed to target subgenus-specific sequences on the multiple-copy 18S rRNA, greatly facilitating the detection of a single trypanosome.

Originele taal-2English
Pagina's (van-tot)4295-4297
Aantal pagina's3
TijdschriftJournal of Clinical Microbiology
Volume40
Nummer van het tijdschrift11
DOI's
StatusPublished - nov 2002

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