DNA polymorphism and epigenetic marks modulate the affinity of a scaffold/matrix attachment region to the nuclear matrix

Natalia P Kisseljova, Petr Dmitriev, Alexey Katargin, Elena Kim, Daria Ezerina, Diana Markozashvili, Daria Malysheva, Emmeline Planche, Richard J L F Lemmers, Silvère M van der Maarel, Dalila Laoudj-Chenivesse, Marc Lipinski, Yegor S Vassetzky

Onderzoeksoutput: Articlepeer review

18 Citaten (Scopus)

Samenvatting

Mechanisms that regulate attachment of the scaffold/matrix attachment regions (S/MARs) to the nuclear matrix remain largely unknown. We have studied the effect of simple sequence length polymorphism (SSLP), DNA methylation and chromatin organization in an S/MAR implicated in facioscapulohumeral dystrophy (FSHD), a hereditary disease linked to a partial deletion of the D4Z4 repeat array on chromosome 4q. This FSHD-related nuclear matrix attachment region (FR-MAR) loses its efficiency in myoblasts from FSHD patients. Three criteria were found to be important for high-affinity interaction between the FR-MAR and the nuclear matrix: the presence of a specific SSLP haplotype in chromosomal DNA, the methylation of one specific CpG within the FR-MAR and the absence of histone H3 acetylated on lysine 9 in the relevant chromatin fragment.

Originele taal-2English
Pagina's (van-tot)1117-1123
Aantal pagina's7
TijdschriftEuropean Journal of Human Genetics
Volume22
Nummer van het tijdschrift9
DOI's
StatusPublished - sep 2014

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