Effect of Phthalates and Their Substitutes on the Physiology of Pseudomonas aeruginosa

Mélissande Louis; Ali Tahrioui; Julien Verdon; Audrey David; Sophie Rodrigues; Magalie Barreau; Maëliss Manac'h; Audrey Thiroux; Baptiste Luton; Charly Dupont; Marie Le Calvé; Alexis Bazire; Alexandre Crépin; Maximilien Clabaut; Emilie Portier; Laure Taupin; Florian Defontaine; Thomas Clamens; Emeline Bouffartigues; Pierre Cornelis; Marc Feuilloley; Jocelyne Caillon; Alain Dufour; Jean-Marc Berjeaud; Olivier Lesouhaitier; Sylvie Chevalier

doi:10.3390/microorganisms10091788

Effect of Phthalates and Their Substitutes on the Physiology of Pseudomonas aeruginosa

Mélissande Louis, Ali Tahrioui, Julien Verdon, Audrey David, Sophie Rodrigues, Magalie Barreau, Maëliss Manac'h, Audrey Thiroux, Baptiste Luton, Charly Dupont, Marie Le Calvé, Alexis Bazire, Alexandre Crépin, Maximilien Clabaut, Emilie Portier, Laure Taupin, Florian Defontaine, Thomas Clamens, Emeline Bouffartigues, Pierre CornelisMarc Feuilloley, Jocelyne Caillon, Alain Dufour, Jean-Marc Berjeaud, Olivier Lesouhaitier, Sylvie Chevalier

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Phthalates are used in a variety of applications-for example, as plasticizers in polyvinylchloride products to improve their flexibility-and can be easily released into the environment. In addition to being major persistent organic environmental pollutants, some phthalates are responsible for the carcinogenicity, teratogenicity, and endocrine disruption that are notably affecting steroidogenesis in mammals. Numerous studies have thus focused on deciphering their effects on mammals and eukaryotic cells. While multicellular organisms such as humans are known to display various microbiota, including all of the microorganisms that may be commensal, symbiotic, or pathogenic, few studies have aimed at investigating the relationships between phthalates and bacteria, notably regarding their effects on opportunistic pathogens and the severity of the associated pathologies. Herein, the effects of phthalates and their substitutes were investigated on the human pathogen, Pseudomonas aeruginosa, in terms of physiology, virulence, susceptibility to antibiotics, and ability to form biofilms. We show in particular that most of these compounds increased biofilm formation, while some of them enhanced the bacterial membrane fluidity and altered the bacterial morphology.

Originele taal-2	English
Artikelnummer	1788
Aantal pagina's	19
Tijdschrift	Microorganisms
Volume	10
Nummer van het tijdschrift	9
DOI's	https://doi.org/10.3390/microorganisms10091788
Status	Published - 5 sep. 2022

Bibliografische nota

Funding Information:
C.D., B.L., M.L.C. and M.M. were supported by a training gratification from the French National Research Program for Environmental and Occupational Health of ANSES (2018/1/223, IMPERIAL). A.T. was supported by a postdoctoral fellowship from the French National Research Program for Environmental and Occupational Heath of ANSES (2018/1/223, IMPERIAL). M.L. was supported by an engineer contract from the ERDF RN-BEE research program (Région Normandie (France). This work was funded by the French National Research Program for Environmental and Occupational Health of ANSES (2018/1/223, IMPERIAL). The CBSA Lab is supported by the Région Normandie (France), the European FEDER funds, and Evreux Portes de Normandie (France). The LBCM is supported by the Région Bretagne (France) and European FEDER.

Publisher Copyright:
© 2022 by the authors.

Copyright:
Copyright 2022 Elsevier B.V., All rights reserved.

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10.3390/microorganisms10091788Licentie: CC BY

Louis et al 2022 MicroorganismsFinal published version, 3,41 MBLicentie: CC BY

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Louis, M., Tahrioui, A., Verdon, J., David, A., Rodrigues, S., Barreau, M., Manac'h, M., Thiroux, A., Luton, B., Dupont, C., Calvé, M. L., Bazire, A., Crépin, A., Clabaut, M., Portier, E., Taupin, L., Defontaine, F., Clamens, T., Bouffartigues, E., ... Chevalier, S. (2022). Effect of Phthalates and Their Substitutes on the Physiology of Pseudomonas aeruginosa. Microorganisms, 10(9), Artikel 1788. https://doi.org/10.3390/microorganisms10091788

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title = "Effect of Phthalates and Their Substitutes on the Physiology of Pseudomonas aeruginosa",

abstract = "Phthalates are used in a variety of applications-for example, as plasticizers in polyvinylchloride products to improve their flexibility-and can be easily released into the environment. In addition to being major persistent organic environmental pollutants, some phthalates are responsible for the carcinogenicity, teratogenicity, and endocrine disruption that are notably affecting steroidogenesis in mammals. Numerous studies have thus focused on deciphering their effects on mammals and eukaryotic cells. While multicellular organisms such as humans are known to display various microbiota, including all of the microorganisms that may be commensal, symbiotic, or pathogenic, few studies have aimed at investigating the relationships between phthalates and bacteria, notably regarding their effects on opportunistic pathogens and the severity of the associated pathologies. Herein, the effects of phthalates and their substitutes were investigated on the human pathogen, Pseudomonas aeruginosa, in terms of physiology, virulence, susceptibility to antibiotics, and ability to form biofilms. We show in particular that most of these compounds increased biofilm formation, while some of them enhanced the bacterial membrane fluidity and altered the bacterial morphology.",

author = "M{\'e}lissande Louis and Ali Tahrioui and Julien Verdon and Audrey David and Sophie Rodrigues and Magalie Barreau and Ma{\"e}liss Manac'h and Audrey Thiroux and Baptiste Luton and Charly Dupont and Calv{\'e}, {Marie Le} and Alexis Bazire and Alexandre Cr{\'e}pin and Maximilien Clabaut and Emilie Portier and Laure Taupin and Florian Defontaine and Thomas Clamens and Emeline Bouffartigues and Pierre Cornelis and Marc Feuilloley and Jocelyne Caillon and Alain Dufour and Jean-Marc Berjeaud and Olivier Lesouhaitier and Sylvie Chevalier",

note = "Funding Information: C.D., B.L., M.L.C. and M.M. were supported by a training gratification from the French National Research Program for Environmental and Occupational Health of ANSES (2018/1/223, IMPERIAL). A.T. was supported by a postdoctoral fellowship from the French National Research Program for Environmental and Occupational Heath of ANSES (2018/1/223, IMPERIAL). M.L. was supported by an engineer contract from the ERDF RN-BEE research program (R{\'e}gion Normandie (France). This work was funded by the French National Research Program for Environmental and Occupational Health of ANSES (2018/1/223, IMPERIAL). The CBSA Lab is supported by the R{\'e}gion Normandie (France), the European FEDER funds, and Evreux Portes de Normandie (France). The LBCM is supported by the R{\'e}gion Bretagne (France) and European FEDER. Publisher Copyright: {\textcopyright} 2022 by the authors. Copyright: Copyright 2022 Elsevier B.V., All rights reserved.",

year = "2022",

month = sep,

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doi = "10.3390/microorganisms10091788",

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Louis, M, Tahrioui, A, Verdon, J, David, A, Rodrigues, S, Barreau, M, Manac'h, M, Thiroux, A, Luton, B, Dupont, C, Calvé, ML, Bazire, A, Crépin, A, Clabaut, M, Portier, E, Taupin, L, Defontaine, F, Clamens, T, Bouffartigues, E, Cornelis, P, Feuilloley, M, Caillon, J, Dufour, A, Berjeaud, J-M, Lesouhaitier, O & Chevalier, S 2022, 'Effect of Phthalates and Their Substitutes on the Physiology of Pseudomonas aeruginosa', Microorganisms, vol. 10, nr. 9, 1788. https://doi.org/10.3390/microorganisms10091788

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T1 - Effect of Phthalates and Their Substitutes on the Physiology of Pseudomonas aeruginosa

AU - Louis, Mélissande

AU - Tahrioui, Ali

AU - Verdon, Julien

AU - David, Audrey

AU - Rodrigues, Sophie

AU - Barreau, Magalie

AU - Manac'h, Maëliss

AU - Thiroux, Audrey

AU - Luton, Baptiste

AU - Dupont, Charly

AU - Calvé, Marie Le

AU - Bazire, Alexis

AU - Crépin, Alexandre

AU - Clabaut, Maximilien

AU - Portier, Emilie

AU - Taupin, Laure

AU - Defontaine, Florian

AU - Clamens, Thomas

AU - Bouffartigues, Emeline

AU - Cornelis, Pierre

AU - Feuilloley, Marc

AU - Caillon, Jocelyne

AU - Dufour, Alain

AU - Berjeaud, Jean-Marc

AU - Lesouhaitier, Olivier

AU - Chevalier, Sylvie

N1 - Funding Information: C.D., B.L., M.L.C. and M.M. were supported by a training gratification from the French National Research Program for Environmental and Occupational Health of ANSES (2018/1/223, IMPERIAL). A.T. was supported by a postdoctoral fellowship from the French National Research Program for Environmental and Occupational Heath of ANSES (2018/1/223, IMPERIAL). M.L. was supported by an engineer contract from the ERDF RN-BEE research program (Région Normandie (France). This work was funded by the French National Research Program for Environmental and Occupational Health of ANSES (2018/1/223, IMPERIAL). The CBSA Lab is supported by the Région Normandie (France), the European FEDER funds, and Evreux Portes de Normandie (France). The LBCM is supported by the Région Bretagne (France) and European FEDER. Publisher Copyright: © 2022 by the authors. Copyright: Copyright 2022 Elsevier B.V., All rights reserved.

PY - 2022/9/5

Y1 - 2022/9/5

N2 - Phthalates are used in a variety of applications-for example, as plasticizers in polyvinylchloride products to improve their flexibility-and can be easily released into the environment. In addition to being major persistent organic environmental pollutants, some phthalates are responsible for the carcinogenicity, teratogenicity, and endocrine disruption that are notably affecting steroidogenesis in mammals. Numerous studies have thus focused on deciphering their effects on mammals and eukaryotic cells. While multicellular organisms such as humans are known to display various microbiota, including all of the microorganisms that may be commensal, symbiotic, or pathogenic, few studies have aimed at investigating the relationships between phthalates and bacteria, notably regarding their effects on opportunistic pathogens and the severity of the associated pathologies. Herein, the effects of phthalates and their substitutes were investigated on the human pathogen, Pseudomonas aeruginosa, in terms of physiology, virulence, susceptibility to antibiotics, and ability to form biofilms. We show in particular that most of these compounds increased biofilm formation, while some of them enhanced the bacterial membrane fluidity and altered the bacterial morphology.

AB - Phthalates are used in a variety of applications-for example, as plasticizers in polyvinylchloride products to improve their flexibility-and can be easily released into the environment. In addition to being major persistent organic environmental pollutants, some phthalates are responsible for the carcinogenicity, teratogenicity, and endocrine disruption that are notably affecting steroidogenesis in mammals. Numerous studies have thus focused on deciphering their effects on mammals and eukaryotic cells. While multicellular organisms such as humans are known to display various microbiota, including all of the microorganisms that may be commensal, symbiotic, or pathogenic, few studies have aimed at investigating the relationships between phthalates and bacteria, notably regarding their effects on opportunistic pathogens and the severity of the associated pathologies. Herein, the effects of phthalates and their substitutes were investigated on the human pathogen, Pseudomonas aeruginosa, in terms of physiology, virulence, susceptibility to antibiotics, and ability to form biofilms. We show in particular that most of these compounds increased biofilm formation, while some of them enhanced the bacterial membrane fluidity and altered the bacterial morphology.

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U2 - 10.3390/microorganisms10091788

DO - 10.3390/microorganisms10091788

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VL - 10

JO - Microorganisms

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ER -

Effect of Phthalates and Their Substitutes on the Physiology of Pseudomonas aeruginosa

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