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This study tends to investigate if non-invasive VNS could suppress RT-induced tumor promoting inflammation and revert the immunosuppressive TME in lung cancer.
Methods Preclinically, lung tumor-bearing C57Bl/6 mice were treated with VNS alone, fractionated RT (2.4 Gy, 4 consecutive days) plus VNS (2x/day, 25HZ, 5 consecutive days) or RT plus sham VNS. Tumor volumes were monitored via bioluminescent imaging. In addition, murine spleens and lungs were subjected to immunological analysis via flow cytometry. Furthermore, NSCLC patients (n=6) were enrolled in a blind randomized clinical trial. Patients were subjected to VNS (2x/day, 20Hz) or sham (control arm) in combination with conventional chemo-RT. Blood was collected in both setups before, during and at the end of treatment for further flow cytometry-based analysis and ELISA.
Preliminary data show that the orthotopic lung cancer model allows evaluation of therapeutic RT efficacy. Furthermore, we observed immune modulation upon VNS, more specifically an increase in conventional type 2 dendritic cells. Accordingly, clinical results show VNS-mediated upregulation of dendritic cells, natural killer cells and CD8+ T cells in blood. In contrast both the granulocytic and monocytic MDSC populations decreased over time.
Though most results are preliminary, we observe trends towards the amelioration of antitumor immunity with reduction of tumor promoting MDSC. VNS is a safe and non-invasive treatment option, therefore it could have a positive impact on (immune)therapy strategies for a broad range of cancer patients.
|Status||Published - 7 feb 2020|
|Evenement||BACR Annual Meeting 2020: Cancer metastasis: From bedside to bench - Vrije Universiteit Brussel, Campus Jette, Brussels, Belgium|
Duur: 7 feb 2020 → 7 feb 2020
|Conference||BACR Annual Meeting 2020|
|Periode||7/02/20 → 7/02/20|
VingerafdrukDuik in de onderzoeksthema's van 'Effects of non-invasive vagal nerve stimulation on radiation-induced inflammation and cancer prognosis'. Samen vormen ze een unieke vingerafdruk.
1/11/17 → 31/10/22
ANI184: Nanobody-targeted radionuclide therapy and immune therapy: a perfect match in the area of combination therapy.
1/01/17 → 31/12/21