Projecten per jaar
Samenvatting
The genome of Trametes versicolor encodes multiple laccase isozymes, the expression of which is responsive to various conditions. Here, we set out to investigate the potential of orange peel extract as an inducer of laccase production in this white-rot fungus, in comparison to the previously identified inducing chemical compound, veratryl alcohol. For four geographically distinct T. versicolor strains, a positive correlation has been observed between their oxidative activity and incubation time in liquid cultures. The addition of 20% orange peel extract or 5 mM veratryl alcohol caused a rapid increase in the oxidative potential of T. versicolor M99 after 24 h, with a more pronounced effect observed for the orange peel extract. To elucidate the underlying molecular mechanisms of the induced laccase activity, a transcriptional gene expression analysis was performed for the seven individual laccase genes in T. versicolor, revealing the upregulation of several laccase genes in response to the addition of each inducer. Notably, the gene encoding TvLac5 demonstrated a substantial upregulation in response to the addition of 20% orange peel extract, likely contributing to the observed increase in its oxidative potential. In conclusion, our results demonstrate that orange peels are a promising agro-industrial side stream for implementation as inducing agents in large-scale laccase production with T. versicolor.
Originele taal-2 | English |
---|---|
Artikelnummer | 370 |
Aantal pagina's | 14 |
Tijdschrift | Journal of Fungi |
Volume | 10 |
Nummer van het tijdschrift | 6 |
DOI's | |
Status | Published - 22 mei 2024 |
Bibliografische nota
Publisher Copyright:© 2024 by the authors.
Vingerafdruk
Duik in de onderzoeksthema's van 'Effects of Orange Peel Extract on Laccase Activity and Gene Expression in Trametes versicolor'. Samen vormen ze een unieke vingerafdruk.-
FWOTM1049: Ontwerp van zelfhelende levende myceliummaterialen op het snijvlak van biologie en materiaalkunde
Vanden Elsacker, E., Peeters, E. & Van den Brande, N.
1/01/23 → 31/12/26
Project: Fundamenteel
-
SRP91: SRP-Groeifinanciering: Synthetische biologie voor de ontwikkeling van microbiële celfabrieken
Peeters, E., Vranken, W. & de Buyl, S.
1/10/22 → 30/09/27
Project: Fundamenteel