Endocrine activity in hospital, WWTP effluent, and river waters from the Brussels region, Belgium using the BG1Luc4E2 CALUX Bioassay.

Kersten Van Langenhove, Tim Reyns, Tara Vandermarken, Pierre Servais, Michael S. Denison, Joris Van Loco, Marc Elskens

Onderzoeksoutput: Meeting abstract (Book)


Endocrine disrupting chemicals (EDCs) are gaining in worldwide attention due to their omnipresence, wide range in chemical properties and possible effects on wildlife populations. Problems with these EDCs are their continuous release into the environment (industrial, domestic, inefficient removal). For this reason, the Water Framework Directive (amended in 2013/39/EU) established a priority list of 33 new and 8 old pollutants and also placed 15 compounds (including estrogens) onto a watch list. Rather than looking solely for concentrations of targeted and well-known EDCs, this project strives to combine bio-analytical and chemo-analytical data on the Zenne river crossing Brussels and hospital effluents (a potential major source for EDCs due to high consumption of pharmaceuticals and personal care products) for the first time. Bioassays allow scientists to use in vitro receptor models to assess endocrine activity by comparing the mixture or cocktail effect originating from the sample extract. Added value is that this technique picks up effects from compounds that are not
routinely monitored in targeted chemo-analytical methods and can detect them at low amounts (pg/L EEQ, Estrogen EQuivalence). Samples are extracted using Oasis HLB SPE columns and analyzed with human ovarian adenocarcinoma (BG1) cells transfected with a luciferase reporter gene. Values ranged from 0.77 to 1.85ng EEQ/L for the Zenne river water, 98ng EEQ/L for hospital effluent and, respectively, 52.3 and 1.2ng EEQ/L for WWTP influent and effluent. For comparison reasons, commercial drinking waters amount to 0.004-0.070 ng EEQ/L. These results indicate that effluent values are high post-discharge of human activities, EDCs are only partially removed by processes in WWTPs, and that effluents contribute significantly to EDC loads in the Zenne river.
Originele taal-2English
TitelChemCYS 2016
Aantal pagina's1
StatusPublished - 16 mrt 2016
EvenementChemistry Conference for Young Scientist: ChemCYS 2016 - Floréal, Blankenberge, Belgium
Duur: 16 mrt 201618 mrt 2016
Congresnummer: 13


ConferenceChemistry Conference for Young Scientist


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