Endocrine activity in hospital, WWTP effluent, and Zenne River waters from the Brussels region, Belgium using the BG1Luc4E2 CALUX bioassay

Kersten Van Langenhove, Tim Reyns, Tara Vandermarken, Servais Pierre, Michael S. Denison, Joris Van Loco, Marc Elskens

Onderzoeksoutput: Meeting abstract (Book)


Endocrine disrupting chemicals (EDCs) are gaining in worldwide attention due to their omnipresence, wide range in chemical properties and possible effects on wildlife populations. Problems with these EDCs are their continuous release into the environment (industrial, domestic, inefficient removal). For this reason, the Water Framework Directive (amended in 2013/39/EU) established a priority list of 33 new and 8 old pollutants and also placed 15 compounds (including estrogens) onto a watch list.

Rather than looking solely for concentrations of targeted and well-known EDCs, this project strives to combine bio-analytical and chemo-analytical data on the Zenne river crossing Brussels and hospital effluents (a potential major source for EDCs due to high consumption of pharmaceuticals and personal care products) for the first time. Bioassays allow scientists to use in vitro receptor models to assess endocrine activity by comparing the mixture or cocktail effect originating from the sample extract.

Samples are extracted using Oasis HLB SPE columns and analyzed with human ovarian adenocarcinoma (BG1) cells transfected with a luciferase reporter gene (BG1Luc4E2 CALUX assay). Over a 12 month sampling period, bioassay values ranged from 0.68 to 16.53ng EEQ/L for the Zenne river water and from 67.40 to 231ng EEQ/L for hospital effluent. WWTP effluent values ranged from 0.96 - 6.62ng EEQ/L and influent levels from 17.6 - 88.8ng EEQ/L. Results indicate that effluent values are high post-discharge of human activities, EDCs are only partially removed by processes in WWTPs, and that effluents can contribute significantly to EDC loads in the Zenne river.
Originele taal-2English
TitelISEAC 39 program
StatusPublished - 18 jul 2016
EvenementISEAC 39: International Symposium on Environmental Analytical Chemistry - University of Hamburg, Geomatikum, Hamburg, Germany
Duur: 18 jul 201622 jul 2016
Congresnummer: 39


ConferenceISEAC 39
Internet adres


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