Endospore Appendages: a novel pilus superfamily from the endospores of pathogenic Bacilli

Brajabandhu Pradhan, Janine Liedtke, Mike Sleutel, Toril Lindbäck, Ephrem Debebe Zegeye, Kristin O Sullivan, Ann-Katrin Llarena, Ola Brynildsrud, Marina Aspholm, Han Remaut

Onderzoeksoutput: Articlepeer review

5 Citaten (Scopus)

Samenvatting

Bacillus cereus sensu lato is a group of Gram-positive endospore-forming bacteria with high ecological diversity. Their endospores are decorated with micrometer-long appendages of unknown identity and function. Here, we isolate endospore appendages (Enas) from the food poisoning outbreak strain B. cereus NVH 0075-95 and find proteinaceous fibers of two main morphologies: S- and L-Ena. By using cryoEM and 3D helical reconstruction of S-Enas, we show these to represent a novel class of Gram-positive pili. S-Enas consist of single domain subunits with jellyroll topology that are laterally stacked by β-sheet augmentation. S-Enas are longitudinally stabilized by disulfide bonding through N-terminal connector peptides that bridge the helical turns. Together, this results in flexible pili that are highly resistant to heat, drought, and chemical damage. Phylogenomic analysis reveals a ubiquitous presence of the ena-gene cluster in the B. cereus group, which include species of clinical, environmental, and food importance. We propose Enas to represent a new class of pili specifically adapted to the harsh conditions encountered by bacterial spores.

Originele taal-2English
Artikelnummere106887
TijdschriftEMBO Journal
Volume40
Nummer van het tijdschrift17
Vroegere onlinedatum25 mei 2021
DOI's
StatusPublished - 1 sep 2021

Bibliografische nota

© 2021 The Authors.

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