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Samenvatting
In Temperature Responsive Liquid Chromatography (TRLC) stimuli-responsive polymers are used to drive the separation processes. This type of polymer is an “intelligent material”, as it is able to respond to a small variation in its surroundings with a sharp change in its physical properties. In the case of temperature responsive polymers, they possess a unique characteristic that allows them to change their water solubility based on changes in the ambient temperature. Implementing this polymer into a bonded-phase for liquid chromatography, allows for the control of the column polarity through control of the column temperature. This introduces the possibility to perform reversed phased like liquid chromatography in pure water, whereby the polarity is controlled through temperature, therefore eliminating the need for any organic modifiers. Although this concept is promising it has not been that straightforward to develop to its full potential, as it requires rapid heating and cooling of the column. Previously, the possibility of this property was already demonstrated by rapidly cooling the column in a water bath. This external cooling of the column, however, leads to the occurrence of radial temperature gradients inside the column, while not allowing for refocusing effect typically associated with the application of gradients. Thus far, although the possibility of TRLC has extensively been demonstrated through several applications, the combination of increased plate height at lower temperatures and of the additional radial temperature gradients inside the column has detrimentally affected chromatographic performance. One of the reasons for this sub-par performance is the absence of an efficient linear gradient setup, which currently limits these types of separations to less efficient and lengthy isothermal measurements.
In this research we will evaluate the possibility of linear temperature gradients in temperature responsive liquid chromatography. For this, a regular HPLC system will be converted into a system that is able to efficiently deliver both hot and cold aqueous mobile phases whilst retaining all of its basic functionalities. In order to achieve the required fast and efficient temperature gradients, an important focus will be placed on achieving efficient heating and cooling of the temperature responsive column whilst minimizing any unwanted heat transfers. After this, the performance of this new setup will be optimized and evaluated by comparison to the current non-linear gradient setup. To demonstrate the capability of this newly developed linear gradient setup, it will then be employed towards the separation of relevant test mixtures, such as food additives and natural products.
In this research we will evaluate the possibility of linear temperature gradients in temperature responsive liquid chromatography. For this, a regular HPLC system will be converted into a system that is able to efficiently deliver both hot and cold aqueous mobile phases whilst retaining all of its basic functionalities. In order to achieve the required fast and efficient temperature gradients, an important focus will be placed on achieving efficient heating and cooling of the temperature responsive column whilst minimizing any unwanted heat transfers. After this, the performance of this new setup will be optimized and evaluated by comparison to the current non-linear gradient setup. To demonstrate the capability of this newly developed linear gradient setup, it will then be employed towards the separation of relevant test mixtures, such as food additives and natural products.
Originele taal-2 | English |
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Status | Published - jan 2020 |
Evenement | 16th International Symposium on Hyphenated Techniques in Chromatography and Separation Technology - Het Pand, Ghent, Belgium Duur: 29 jan 2020 → 31 jan 2020 https://kuleuvencongres.be/htc16 |
Conference
Conference | 16th International Symposium on Hyphenated Techniques in Chromatography and Separation Technology |
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Verkorte titel | HTC-16 |
Land/Regio | Belgium |
Stad | Ghent |
Periode | 29/01/20 → 31/01/20 |
Internet adres |
Vingerafdruk
Duik in de onderzoeksthema's van 'Enhancing the Application and Exploitation of Temperature Gradients in Temperature Responsive Liquid Chromatography'. Samen vormen ze een unieke vingerafdruk.Projecten
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SRP6: SRP (Zwaartepunt): exploitatie van de voordelen van de Orde in Opsluiting voor een groenere chemie
Desmet, G., Denayer, J., Denayer, J., Desmet, G. & Denayer, J.
1/11/12 → 31/10/22
Project: Fundamenteel