Samenvatting

The current state-of-the-art UHPLC technology comprises instrumentation allowing to operate columns packed with 1.5 um core-shell particles at operating pressures up to 1500 bar. To effectively utilize the potential of these columns, yielding peak volumes ranging between 0.5 and 10 uL, extra-column dispersion contributions need to be minimized.

In the current study, the instrument configuration of a UHPLC system with 1500 bar pressure capabilities was systematically altered and its effects on separation efficiency and retention were assessed in both the isocratic and gradient modes. Key factors affecting extra-column dispersion were investigated and the optimal system configuration with respect to extra-column dispersion and flow resistance is discussed for isocratic and gradient LC mode. Furthermore, pressure-induced retention effects have been evaluated, which affect method development and HPLC to UHPLC method-transfer strategies.
Originele taal-2English
Pagina's63-63
Aantal pagina's1
StatusPublished - jan 2020
Evenement16th International Symposium on Hyphenated Techniques in Chromatography and Separation Technology - Het Pand, Ghent, Belgium
Duur: 29 jan 202031 jan 2020
https://kuleuvencongres.be/htc16

Conference

Conference16th International Symposium on Hyphenated Techniques in Chromatography and Separation Technology
Verkorte titelHTC-16
LandBelgium
StadGhent
Periode29/01/2031/01/20
Internet adres

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