Fast and Accurate Identification of Dermatophytes by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry: Validation in the Clinical Laboratory

Ann Packeu; Annelies De Bel; C. Weil-Olivier; S Ranque; M Detandt; M Hendrickx

doi:10.1128/JCM.01428-14

Fast and Accurate Identification of Dermatophytes by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry: Validation in the Clinical Laboratory

Ann Packeu
, Annelies De Bel
, C. Weil-Olivier
, S Ranque
, M Detandt
, M Hendrickx

Immunologie en Microbiologie

Onderzoeksoutput: Article › peer review

43 Citaten (Scopus)

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The performance of a matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) workflow using an extensive reference database for dermatophyte identification was evaluated on 176 clinical strains. Using a direct-deposit procedure after 3 incubation days yielded 40% correct identification. Both increasing incubation time and using an extraction procedure resulted in 100% correct identification.

Originele taal-2	English
Pagina's (van-tot)	3440-3443
Aantal pagina's	4
Tijdschrift	Journal of Clinical Microbiology
Volume	52
Nummer van het tijdschrift	9
DOI's	https://doi.org/10.1128/JCM.01428-14
Status	Published - sep. 2014

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title = "Fast and Accurate Identification of Dermatophytes by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry: Validation in the Clinical Laboratory",

abstract = "The performance of a matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) workflow using an extensive reference database for dermatophyte identification was evaluated on 176 clinical strains. Using a direct-deposit procedure after 3 incubation days yielded 40\% correct identification. Both increasing incubation time and using an extraction procedure resulted in 100\% correct identification.",

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AU - Ranque, S

AU - Detandt, M

AU - Hendrickx, M

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AB - The performance of a matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) workflow using an extensive reference database for dermatophyte identification was evaluated on 176 clinical strains. Using a direct-deposit procedure after 3 incubation days yielded 40% correct identification. Both increasing incubation time and using an extraction procedure resulted in 100% correct identification.

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U2 - 10.1128/JCM.01428-14

DO - 10.1128/JCM.01428-14

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Fast and Accurate Identification of Dermatophytes by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry: Validation in the Clinical Laboratory

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