FT4 immunoassays may display a pattern during pregnancy similar to the equilibrium dialysis ID-LC/tandem MS candidate reference measurement procedure in spite of susceptibility towards binding protein alterations.

Ellen Anckaert; Kris Poppe; K. Van Uytfanghe; Johan Schiettecatte; Walter Foulon; L. Thienpont

FT4 immunoassays may display a pattern during pregnancy similar to the equilibrium dialysis ID-LC/tandem MS candidate reference measurement procedure in spite of susceptibility towards binding protein alterations.

Ellen Anckaert, Kris Poppe, K. Van Uytfanghe, Johan Schiettecatte, Walter Foulon, L. Thienpont

Onderzoeksoutput: Article › peer review

68 Citaten (Scopus)

Samenvatting

BACKGROUND: Serum free thyroxine (FT4) testing in pregnancy is known to be challenging for immunoassays (IAs). We verified the reliability of FT4 results by 3 commercial IAs throughout pregnancy, by comparison of the pattern to that obtained with an equilibrium dialysis isotope dilution-mass spectrometry (ED ID-MS) candidate reference measurement procedure.

METHODS: Pregnant females (107) and age-matched non-pregnant controls (26) were enrolled. The IAs tested were those performed on the Cobas 6000 (Roche Diagnostics), ARCHITECT i2000SR (Abbott Diagnostics) and Immulite 2000 (Siemens Healthcare) platforms.

RESULTS: Compared to the controls (mean FT4: 18.2pmol/L), ED ID-MS gave in the late first trimester pregnancy an 8.8% lower (p
CONCLUSION: We proved that IAs may give a FT4 pattern during pregnancy similar to that obtained by ED ID-MS.

Originele taal-2	English
Pagina's (van-tot)	1348-1353
Aantal pagina's	6
Tijdschrift	Clin Chim Acta
Volume	411
Nummer van het tijdschrift	September
Status	Published - sep 2010

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http://www.ncbi.nlm.nih.gov/pubmed/20510682

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Anckaert, E., Poppe, K., Van Uytfanghe, K., Schiettecatte, J., Foulon, W., & Thienpont, L. (2010). FT4 immunoassays may display a pattern during pregnancy similar to the equilibrium dialysis ID-LC/tandem MS candidate reference measurement procedure in spite of susceptibility towards binding protein alterations. Clin Chim Acta, 411(September), 1348-1353. http://www.ncbi.nlm.nih.gov/pubmed/20510682

Anckaert, Ellen ; Poppe, Kris ; Van Uytfanghe, K. ; Schiettecatte, Johan ; Foulon, Walter ; Thienpont, L. / FT4 immunoassays may display a pattern during pregnancy similar to the equilibrium dialysis ID-LC/tandem MS candidate reference measurement procedure in spite of susceptibility towards binding protein alterations. In: Clin Chim Acta. 2010 ; Vol. 411, Nr. September. blz. 1348-1353.

@article{c625a44cee094e18a3a3a37ae6f02b49,

title = "FT4 immunoassays may display a pattern during pregnancy similar to the equilibrium dialysis ID-LC/tandem MS candidate reference measurement procedure in spite of susceptibility towards binding protein alterations.",

abstract = "BACKGROUND: Serum free thyroxine (FT4) testing in pregnancy is known to be challenging for immunoassays (IAs). We verified the reliability of FT4 results by 3 commercial IAs throughout pregnancy, by comparison of the pattern to that obtained with an equilibrium dialysis isotope dilution-mass spectrometry (ED ID-MS) candidate reference measurement procedure. METHODS: Pregnant females (107) and age-matched non-pregnant controls (26) were enrolled. The IAs tested were those performed on the Cobas 6000 (Roche Diagnostics), ARCHITECT i2000SR (Abbott Diagnostics) and Immulite 2000 (Siemens Healthcare) platforms. RESULTS: Compared to the controls (mean FT4: 18.2pmol/L), ED ID-MS gave in the late first trimester pregnancy an 8.8% lower (p CONCLUSION: We proved that IAs may give a FT4 pattern during pregnancy similar to that obtained by ED ID-MS.",

keywords = "FT4 immunoassays, pregnancy",

author = "Ellen Anckaert and Kris Poppe and {Van Uytfanghe}, K. and Johan Schiettecatte and Walter Foulon and L. Thienpont",

year = "2010",

month = sep,

language = "English",

volume = "411",

pages = "1348--1353",

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Anckaert, E, Poppe, K, Van Uytfanghe, K, Schiettecatte, J, Foulon, W & Thienpont, L 2010, 'FT4 immunoassays may display a pattern during pregnancy similar to the equilibrium dialysis ID-LC/tandem MS candidate reference measurement procedure in spite of susceptibility towards binding protein alterations.', Clin Chim Acta, vol. 411, nr. September, blz. 1348-1353. <http://www.ncbi.nlm.nih.gov/pubmed/20510682>

FT4 immunoassays may display a pattern during pregnancy similar to the equilibrium dialysis ID-LC/tandem MS candidate reference measurement procedure in spite of susceptibility towards binding protein alterations. / Anckaert, Ellen; Poppe, Kris; Van Uytfanghe, K.; Schiettecatte, Johan; Foulon, Walter; Thienpont, L.

In: Clin Chim Acta, Vol. 411, Nr. September, 09.2010, blz. 1348-1353.

Onderzoeksoutput: Article › peer review

TY - JOUR

T1 - FT4 immunoassays may display a pattern during pregnancy similar to the equilibrium dialysis ID-LC/tandem MS candidate reference measurement procedure in spite of susceptibility towards binding protein alterations.

AU - Anckaert, Ellen

AU - Poppe, Kris

AU - Van Uytfanghe, K.

AU - Schiettecatte, Johan

AU - Foulon, Walter

AU - Thienpont, L.

PY - 2010/9

Y1 - 2010/9

N2 - BACKGROUND: Serum free thyroxine (FT4) testing in pregnancy is known to be challenging for immunoassays (IAs). We verified the reliability of FT4 results by 3 commercial IAs throughout pregnancy, by comparison of the pattern to that obtained with an equilibrium dialysis isotope dilution-mass spectrometry (ED ID-MS) candidate reference measurement procedure. METHODS: Pregnant females (107) and age-matched non-pregnant controls (26) were enrolled. The IAs tested were those performed on the Cobas 6000 (Roche Diagnostics), ARCHITECT i2000SR (Abbott Diagnostics) and Immulite 2000 (Siemens Healthcare) platforms. RESULTS: Compared to the controls (mean FT4: 18.2pmol/L), ED ID-MS gave in the late first trimester pregnancy an 8.8% lower (p CONCLUSION: We proved that IAs may give a FT4 pattern during pregnancy similar to that obtained by ED ID-MS.

AB - BACKGROUND: Serum free thyroxine (FT4) testing in pregnancy is known to be challenging for immunoassays (IAs). We verified the reliability of FT4 results by 3 commercial IAs throughout pregnancy, by comparison of the pattern to that obtained with an equilibrium dialysis isotope dilution-mass spectrometry (ED ID-MS) candidate reference measurement procedure. METHODS: Pregnant females (107) and age-matched non-pregnant controls (26) were enrolled. The IAs tested were those performed on the Cobas 6000 (Roche Diagnostics), ARCHITECT i2000SR (Abbott Diagnostics) and Immulite 2000 (Siemens Healthcare) platforms. RESULTS: Compared to the controls (mean FT4: 18.2pmol/L), ED ID-MS gave in the late first trimester pregnancy an 8.8% lower (p CONCLUSION: We proved that IAs may give a FT4 pattern during pregnancy similar to that obtained by ED ID-MS.

KW - FT4 immunoassays

KW - pregnancy

M3 - Article

VL - 411

SP - 1348

EP - 1353

JO - Clinica Chimica Acta

JF - Clinica Chimica Acta

SN - 0009-8981

IS - September

ER -