Functional expression of agrobacterium tumefaciens T-DNA onc-genes in asparagus crown gall tissues

Els Prinsen, Benny Bytebier, Jean Pierre Hernalsteens, Jan De Greef, Henri Van Onckelen

Onderzoeksoutput: Articlepeer review

17 Citaten (Scopus)

Samenvatting

We analyzed Asparagus crown gall tissues transformed with A. tumefaciens C58 and C58C1 (pTiB6S3) and selected for hormone autotrophic growth. No increased IAA levels were observed in the Asparagus tumor lines notwithstanding the presence of all three T-DNA onc genes. The endogenous cytokinin levels indicate that Asparagus crown gall is dependent on enhanced zeatin riboside equivalent levels for its growth.We conclude that phytohormone autotrophic growth of Asparagus crown gall tissue seems only to be dependent upon an active gene 4, inducing enhanced cytokinin levels. Moreover, the presence of an active gene 1 seems to be lethal as was indicated by the absence of tryptophan-2-mono-oxygenase activity in transformed tissues and the toxicity of exogenously supplied indole-3-acetamide (IAM) or naphthalene-1-acetamide (NAM) as a substitute for an active gene 1.

Originele taal-2English
Pagina's (van-tot)69-75
Aantal pagina's7
TijdschriftPlant and Cell Physiology
Volume31
Nummer van het tijdschrift1
StatusPublished - 1 dec 1990

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