Genetic ablation of pannexin1 counteracts liver fibrosis in a chemical, but not in a surgical mouse model

Sara Crespo Yanguas, Tereza Cristina da Silva, Isabel Veloso Alves Pereira, Michaël Maes, Joost Willebrords, Valery I Shestopalov, Bruna Goes, Marina Sayuri Nogueira, Inar A. de Castro, Guilherme R. Romualdo, Luis F. Barbisan, Eva Gijbels, Mathieu Vinken, Bruno Cogliati

Onderzoeksoutput: Articlepeer review

11 Citaten (Scopus)
149 Downloads (Pure)

Samenvatting

Liver fibrosis is the final common pathway for almost all causes of chronic liver injury. This chronic disease is characterized by excessive deposition of extracellular matrix components mainly due to transdifferentiation of quiescent hepatic stellate cell into myofibroblasts-like cells, which in turn is driven by cell death and inflammation. In the last few years, paracrine signaling through pannexin1 channels has emerged as a key player in the latter processes. The current study was set up to investigate the role of pannexin1 signaling in liver fibrosis. Wild-type and whole body pannexin1 knock-out mice were treated with carbon tetrachloride or subjected to bile duct ligation. Evaluation of the effects of pannexin1 deletion was based on a number of clinically relevant read-outs, including markers of liver damage, histopathological analysis, oxidative stress, inflammation and regenerative capacity. In parallel, to elucidate the molecular pathways affected by pannexin1 deletion as well as to mechanistically anchor the clinical observations, whole transcriptome analysis of liver tissue was performed. While pannexin1 knock-out mice treated with carbon tetrachloride displayed reduced collagen content, hepatic stellate cell activation, inflammation and hepatic regeneration, bile duct ligated counterparts showed increased hepatocellular injury and antioxidant enzyme activity with a predominant immune response. Gene expression profiling revealed a downregulation of fibrotic and immune responses in pannexin1 knock-out mice treated with carbon tetrachloride, whereas bile duct ligated pannexin1-deficient animals showed a pronounced inflammatory profile. This study shows for the first time an etiology-dependent role for pannexin1 signaling in experimental liver fibrosis.
Originele taal-2English
Pagina's (van-tot)2607-2627
Aantal pagina's21
TijdschriftArchives of Toxicology
Volume92
Nummer van het tijdschrift8
DOI's
StatusPublished - 1 aug 2018

Vingerafdruk

Duik in de onderzoeksthema's van 'Genetic ablation of pannexin1 counteracts liver fibrosis in a chemical, but not in a surgical mouse model'. Samen vormen ze een unieke vingerafdruk.

Citeer dit