Genetic lineage tracing of beta cell neogenesis

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Samenvatting

Genetic lineage tracing is an invaluable tool to demonstrate and measure neogenesis of beta cells from putative precursor cells. Cre-Lox recombination technology can be used for indelible labelling of a cohort of cells and following the fate of these cells and their progeny in animal models. Here, the combination is described of beta-galactosidase enzymatic staining with immunohistochemical staining to demonstrate labelled cells. This technique is performed in tissue cryosections.
Originele taal-2English
TitelAnimal models in diabetes research
RedacteurenHans-georg Joost, Hadi Al-hasani, Annette Schürmann
UitgeverijHumana Press, Springer
Pagina's317-322
Aantal pagina's6
ISBN van elektronische versie978-1-62703-068-7
ISBN van geprinte versie978-1-62703-067-0
StatusPublished - 2012

Publicatie series

NaamMethods in Molecular Biology
Volume933
ISSN van geprinte versie1064-3745

Bibliografische nota

Hans-Georg Joost, Hadi Al-Hasani, Annette Schürmann

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