Global genome diversity of the Leishmania donovani complex

Susanne U Franssen, Caroline Durrant, Olivia Stark, Bettina Moser, Tim Downing, Hideo Imamura, Jean-Claude Dujardin, Mandy J Sanders, Isabel Mauricio, Michael A Miles, Lionel F Schnur, Charles L Jaffe, Abdelmajeed Nasereddin, Henk Schallig, Matthew Yeo, Tapan Bhattacharyya, Mohammad Z Alam, Matthew Berriman, Thierry Wirth, Gabriele SchönianJames A Cotton

Onderzoeksoutput: Articlepeer review

80 Citaten (Scopus)

Samenvatting

Protozoan parasites of the Leishmania donovani complex – L. donovani and L. infantum – cause the fatal disease visceral leishmaniasis. We present the first comprehensive genome-wide global study, with 151 cultured field isolates representing most of the geographical distribution. L. donovani isolates separated into five groups that largely coincide with geographical origin but vary greatly in diversity. In contrast, the majority of L. infantum samples fell into one globally-distributed group with little diversity. This picture is complicated by several hybrid lineages. Identified genetic groups vary in heterozygosity and levels of linkage, suggesting different recombination histories. We characterise chromosome-specific patterns of aneuploidy and identified extensive structural variation, including known and suspected drug resistance loci. This study reveals greater genetic diversity than suggested by geographically-focused studies, provides a resource of genomic variation for future work and sets the scene for a new understanding of the evolution and genetics of the Leishmania donovani complex.

Originele taal-2English
Artikelnummere51243
TijdschrifteLife
Volume9
DOI's
StatusPublished - 25 mrt 2020

Bibliografische nota

Funding Information:
We are very grateful to Gad Baneth from the Koret School of Veterinary Medicine, Hebrew University of Jerusalem, Israel; Patrick Bastien from the Centre National de Référence des Leishmanioses Montpellier, France; Sayda Hassan El Safi from Khartoum University, Khartoum, Sudan; Olga Francino Martí from Universitat Auònoma de Barcelona, Spain; Marina Gramiccia from the Infectious Diseases Department Istituto Superiore di Sanità, Rome, Italy; Ketty Soteriadou and Evi Gouzelou from the Hellenic Pasteur Institute Athens, Greece; A.K.M. Shamsuzzaman from Mymensingh Medical College and Be-Nazir Ahmed from Institute of Epidemiology, Disease Control and Research, Bangladesh; Vanessa Yardley from the London School of Hygiene and Tropical Medicine and Peter Walden from Charité Universitätsmedizin Berlin, Germany, each for providing isolates for this study. We also thank the Leishmania Collection of the Oswaldo Cruz Foundation, Brazil and Elisa Cupolillo for providing the isolates MHOM/BR/2003/MAM, MHOM/BR/2007/ARL, MHOM/BR/2007/WC, voucher numbers: IOC/L2651, IOC/L2935 and IOC/L3015, respectively. Further details of collaborators and their donated samples are given in Supplementary file 1. This project was supported by Wellcome through its core funding of the Wellcome Sanger Institute (grants WT098051 and WT206194) and by the EU framework program FP7-222895.

Funding Information:
This project was supported by Wellcome through its core funding of the Wellcome Sanger Institute (grants WT098051 and WT206194) and by the EU framework program FP7-222895.

Publisher Copyright:
© Franssen et al.

Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.

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