Heterogeneity of the gut microbiome in mice: guidelines for optimizing experimental design

D Laukens, B M Brinkman, Jeroen Raes, M De Vos, P Vandenabeele

Onderzoeksoutput: Articlepeer review

282 Citaten (Scopus)

Samenvatting

Targeted manipulation of the gut flora is increasingly being recognized as a means to improve human health. Yet, the temporal dynamics and intra- and interindividual heterogeneity of the microbiome represent experimental limitations, especially in human cross-sectional studies. Therefore, rodent models represent an invaluable tool to study the host-microbiota interface. Progress in technical and computational tools to investigate the composition and function of the microbiome has opened a new era of research and we gradually begin to understand the parameters that influence variation of host-associated microbial communities. To isolate true effects from confounding factors, it is essential to include such parameters in model intervention studies. Also, explicit journal instructions to include essential information on animal experiments are mandatory. The purpose of this review is to summarize the factors that influence microbiota composition in mice and to provide guidelines to improve the reproducibility of animal experiments.
Originele taal-2English
Pagina's (van-tot)117-132
Aantal pagina's15
TijdschriftFEMS Microbiology Reviews
Volume38
Nummer van het tijdschrift6
DOI's
StatusPublished - 30 aug 2015

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