Heterologous expression, purification and characterisation of the extracellular domain of trypanosome invariant surface glycoprotein ISG75

Engineering & Materials Science

• Affinity chromatography 56%
• Animals 28%
• Arginine 51%
• Characterization (materials science) 51%
• Circular dichroism spectroscopy 60%
• Conformations 36%
• Escherichia coli 37%
• Glutathione 53%
• Glycoproteins 100%
• Liquid chromatography 44%
• Mass spectrometry 37%
• Messenger RNA 41%
• Oxidoreductases 88%
• Parasites 42%
• Proteins 53%
• Purification 65%
• Transfer RNA 48%

Medicine & Life Sciences

• Affinity Chromatography 31%
• Animals 14%
• Arginine 27%
• Buffers 27%
• Circular Dichroism 35%
• Complementary DNA 23%
• Cytoplasm 24%
• Disulfides 31%
• Escherichia coli 20%
• Glycoproteins 25%
• Histidine 32%
• Immune Sera 26%
• Mass Spectrometry 26%
• Membrane Glycoproteins 66%
• Oxidoreductases 26%
• Parasites 24%
• Plasmids 23%
• Protein Biosynthesis 31%
• Proteins 21%
• Rabbits 20%
• Reverse-Phase Chromatography 36%
• Routine Diagnostic Tests 27%
• Serum 13%
• Spectrum Analysis 28%
• thioredoxin glutathione reductase 55%
• Transfer RNA 32%
• Trypanosoma 73%
• Trypanosoma brucei brucei 38%

Chemistry

• Affinity Chromatography 28%
• Buffer Solution 18%
• Circular Dichroism 23%
• Complementary DNA 30%
• Disulfide 24%
• Glutathione 26%
• Glycoprotein 53%
• Histidine 27%
• Liquid Chromatography 21%
• Mass Spectrometry 19%
• Protein 24%
• Purification 39%
• Purity 14%
• Strain 20%
• Surface 18%
• Thioredoxin 37%