In vivo transfer of the Ti-plasmid of Agrobacterium tumefaciens to Escherichia coli

M. Holsters, B. Silva, F. Van Vliet, J. P. Hernalsteens, C. Genetello, M. Van Montagu, J. Schell

Onderzoeksoutput: Articlepeer review

22 Citaten (Scopus)

Samenvatting

The Ti-plasmids are naturally self-transmissible from their normal host Agrobacterium to E. coli. They are however unable to stably establish themselves as a replicon in E. coli. It is nevertheless possible to study the Ti-plasmids in E. coli with the help of Ti::RP4 cointegrate plasmids that transfer and maintain themselves very efficiently in E. coli. An E. coli harbouring such a Ti::RP4 plasmid is unable to catabolize octopine and unable to induce crowngall tumours on plants.

Originele taal-2English
Pagina's (van-tot)335-338
Aantal pagina's4
TijdschriftMGG Molecular & General Genetics
Volume163
Nummer van het tijdschrift3
DOI's
StatusPublished - 1 jan 1978

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