Inflammation Alters the Secretome and Immunomodulatory Properties of Human Skin-Derived Precursor Cells

Joery De Kock, Robim Marcelino Rodrigues, Steven Branson, Lieven Verhoye, Haaike Colemonts-Vroninks, Matthias Rombaut, Joost Boeckmans, Jessie Neuckermans, Sien Lequeue, Karolien Buyl, Makram Merimi, Douaa Moussa Agha, Veerle De Boe, Laurence Lagneaux, Philip Meuleman, Tamara Vanhaecke, Mehdi Najar

Onderzoeksoutput: Articlepeer review

6 Citaten (Scopus)
63 Downloads (Pure)


Human skin-derived precursors (SKP) represent a group of somatic stem/precursor cells that reside in dermal skin throughout life that harbor clinical potential. SKP have a high self-renewal capacity, the ability to differentiate into multiple cell types and low immunogenicity, rendering them key candidates for allogeneic cell-based, off-the-shelf therapy. However, potential clinical application of allogeneic SKP requires that these cells retain their therapeutic properties under all circumstances and, in particular, in the presence of an inflammation state. Therefore, in this study, we investigated the impact of pro-inflammatory stimulation on the secretome and immunosuppressive properties of SKP. We demonstrated that pro-inflammatory stimulation of SKP significantly changes their expression and the secretion profile of chemo/cytokines and growth factors. Most importantly, we observed that pro-inflammatory stimulated SKP were still able to suppress the graft-versus-host response when cotransplanted with human PBMC in severe-combined immune deficient (SCID) mice, albeit to a much lesser extent than unstimulated SKP. Altogether, this study demonstrates that an inflammatory microenvironment has a significant impact on the immunological properties of SKP. These alterations need to be taken into account when developing allogeneic SKP-based therapies.

Originele taal-2English
Aantal pagina's17
Nummer van het tijdschrift4
StatusPublished - 8 apr 2020

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© 2020 by the authors. Licensee MDPI, Basel, Switzerland.


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