Insertional mutagenesis in Arabidopsis thaliana

M. Van Lijsebettens, B. den Boer, J. P. Hernalsteens, M. Van Montagu

Onderzoeksoutput: Articlepeer review

10 Citaten (Scopus)

Samenvatting

Recently, a number of mutant gene loci in the Arabidopsis thaliana plant genome have been identified through insertional mutagenesis. In this review, we evaluate different methods used for Agrobacterium tumefaciens-mediated T-DNA insertional mutagenesis with regard to their mutation frequencies and conclude that a major breakthrough in the isolation of genes involved in plant development has been acheived. To provide a specific example, we summarize recent progress made in the understanding of flower morphogenesis at the molecular level through the study of homeotic genes obtained via gene tagging. T-DNA gene fusion vectors are being discussed that will allow the isolation of plant regulatory sequences with particular cell or tissue specificity, or that are controlled by specific external stimuli. Finally, we report on the approaches followed to convert the maize transposons Ac/Ds into valuable gene tags for use in a heterologous host such as Arabidopsis.

Originele taal-2English
Pagina's (van-tot)27-37
Aantal pagina's11
TijdschriftPlant Science
Volume80
Nummer van het tijdschrift1-2
DOI's
StatusPublished - 1 jan 1991

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