Introduction and expression of the octopine T-DNA oncogenes in tobacco plants and their progeny

Francoise Budar, Francine Deboeck, Marc Van Montagu, Jean-Pierre Hernálsteens

Onderzoeksoutput: Articlepeer review

25 Citaten (Scopus)

Samenvatting

The tumour-inducing T-DNA genes 1, 2 and 4 of the octopine Ti-plasmid pTiAch5 were cloned and introduced into tobacco cells by cocultivation or leaf disk transformation using pTi derived vectors. When a selectable marker was needed, we used a aminoglycoside phosphotransferase II (nos-APH(3′)II) chimeric gene conferring kanamycin resistance to plant cells. The expression of gene 4 in transformed tissue cultures precluded the regeneration of normal transformed plants. Normal transformed plants were obtained with the construction carrying genes 1 or 2. We report in vivo complementation of genes 1 and 2 after crosses of transformed plants. Strategies are described for the use of genes 1 and 2 as selection or screening markers in plant cells or regenerated plants.

Originele taal-2English
Pagina's (van-tot)195-206
Aantal pagina's12
TijdschriftPlant Science
Volume46
Nummer van het tijdschrift3
DOI's
StatusPublished - 1 jan 1986

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