Light triggered nanoscale biolistics for efficient intracellular delivery of functional macromolecules in mammalian cells

Juan C Fraire, Elnaz Shaabani, Maryam Sharifiaghdam, Matthias Rombaut, Charlotte Hinnekens, Dawei Hua, Jana Ramon, Laurens Raes, Eduardo Bolea-Fernandez, Toon Brans, Frank Vanhaecke, Peter Borghgraef, Chaobo Huang, Félix Sauvage, Tamara Vanhaecke, Joery De Kock, Ranhua Xiong, Stefaan De Smedt, Kevin Braeckmans

Onderzoeksoutput: Articlepeer review

3 Citaten (Scopus)

Samenvatting

Biolistic intracellular delivery of functional macromolecules makes use of dense microparticles which are ballistically fired onto cells with a pressurized gun. While it has been used to transfect plant cells, its application to mammalian cells has met with limited success mainly due to high toxicity. Here we present a more refined nanotechnological approach to biolistic delivery with light-triggered self-assembled nanobombs (NBs) that consist of a photothermal core particle surrounded by smaller nanoprojectiles. Upon irradiation with pulsed laser light, fast heating of the core particle results in vapor bubble formation, which propels the nanoprojectiles through the cell membrane of nearby cells. We show successful transfection of both adherent and non-adherent cells with mRNA and pDNA, outperforming electroporation as the most used physical transfection technology by a factor of 5.5-7.6 in transfection yield. With a throughput of 104-105 cells per second, biolistic delivery with NBs offers scalable and highly efficient transfections of mammalian cells.

Originele taal-2English
Artikelnummer1996
Aantal pagina's16
TijdschriftNature Communications
Volume13
Nummer van het tijdschrift1
DOI's
StatusPublished - dec 2022

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© 2022. The Author(s).

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