Gene editing in the murine germline is a valuable approach to investigate germ cell maturation and generate mouse models. Several studies demonstrated that CRISPR/Cas9 alters the genome of cultured male mouse germline stem cells delivered by electroporation of plasmids. Recently, we showed proof-of-principle that gene knockout can be effectively targeted in mouse germline stem cells by lipofecting Cas9:gRNA ribonucleoproteins. In this protocol, we describe a simple, fast, and cheap workflow for gene editing via the lipofection of non-integrative ribonucleoproteins in murine male germline stem cells.

Originele taal-2English
Pagina's (van-tot)123-134
Aantal pagina's12
TijdschriftMethods in molecular biology (Clifton, N.J.)
StatusPublished - 2024

Bibliografische nota

© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.


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