Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Rapid Detection of Isolates Belonging to the Epidemic Clones Achromobacter xylosoxidans ST137 and Achromobacter ruhlandii DES from Cystic Fibrosis Patients

Thomas Garrigos, Manon Dollat, Arnaud Magallon, Angélique Chapuis, Véronique Varin, Julien Bador, Nadia Makki, Lise Cremet, Elise Persyn, Emilie Cardot-Martin, Fedoua Echahidi, Charlotte Peeters, Denis Pierard, Peter Vandamme, Alexia Verroken, Catherine Neuwirth, Lucie Amoureux

Onderzoeksoutput: Articlepeer review

2 Citaten (Scopus)

Samenvatting

Achromobacter spp. are increasingly reported among cystic fibrosis patients. Genotyping requires time-consuming methods such as multilocus sequence typing or pulsed-field gel electrophoresis. Therefore, data on the prevalence of multiresistant epidemic clones, especially A. xylosoxidans ST137 (AxST137) and the Danish epidemic strain A. ruhlandii (DES), are lacking. We recently developed and published a database for Achromobacter species identification by matrix-assisted laser desorption-ionization-time of flight mass spectrometry (MALDI-TOF MS; Bruker Daltonics). The aim of this study was to evaluate the ability of the MALDI-TOF MS to distinguish these multiresistant epidemic clones within Achromobacter species. All the spectra of A. xylosoxidans (n = 1,571) and A. ruhlandii (n = 174) used to build the local database were analyzed by ClinProTools, MALDI Biotyper PCA, MALDI Biotyper dendrogram, and flexAnalysis software for biomarker peak detection. Two hundred two isolates (including 48 isolates of AxST137 and 7 of DES) were tested. Specific biomarker peaks were identified: absent peak at m/z 6,651 for AxST137 isolates and present peak at m/z 9,438 for DES isolates. All tested isolates were well typed by our local database and clustered within distinct groups (ST137 or non-ST137 and DES or non-DES) no matter the MALDI-TOF software or only by simple visual inspection of the spectra by any user. The use of MALDI-TOF MS allowed us to identify isolates of A. xylosoxidans belonging to the AxST137 clone that spread in France and Belgium (the Belgian epidemic clone) and of A. ruhlandii belonging to the DES clone. This tool will help the implementation of segregation measures to avoid interpatient transmission of these resistant clones.

Originele taal-2English
Artikelnummere00946
TijdschriftJournal of Clinical Microbiology
Volume59
Nummer van het tijdschrift10
DOI's
StatusPublished - okt 2021

Bibliografische nota

Funding Information:
We thank Didier Hocquet and Marlène Sauget for their advice on the use of ClinProTools and Niels Nørskov-Lauritsen for providing strains of A. ruhlandii DES.

Publisher Copyright:
© 2021 American Society for Microbiology. All Rights Reserved.

Copyright:
Copyright 2023 Elsevier B.V., All rights reserved.

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