Measuring Early Germ-Layer Specification Bias in Human Pluripotent Stem Cells

Alexander Keller; Nuša Krivec; Christina Markouli; Claudia Spits

doi:10.1007/978-1-0716-1979-7_5

Measuring Early Germ-Layer Specification Bias in Human Pluripotent Stem Cells

Alexander Keller, Nuša Krivec, Christina Markouli, Claudia Spits

Onderzoeksoutput: Article › peer review

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Human pluripotent stem cells have a wide variety of potential applications, ranging from clinical translation to in vitro disease modeling. However, there is significant variation in the potential of individual cell lines to differentiate towards each of the three germ layers as a result of (epi)genetic background, culture conditions, and other factors. We describe here in detail a methodology to evaluate this bias using short directed differentiation towards neuroectoderm, mesendoderm, and definitive endoderm in combination with quantification by RT-qPCR and immunofluorescent stains.

Originele taal-2	English
Pagina's (van-tot)	57-72
Aantal pagina's	16
Tijdschrift	Methods in Molecular Biology
Volume	2429
DOI's	https://doi.org/10.1007/978-1-0716-1979-7_5
Status	Published - 2022

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10.1007/978-1-0716-1979-7_5

Spits_PAB
https://www.springernature.com/gp/open-research/policies/book-policies
Accepted author manuscript, 4,28 MBLicentie: Unspecified

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Link to publication in Scopus

FWOTM1016: De impact van recurrente chromosomale afwijkingen op het groeivoordeel en de aanleg tot differentiatie van menselijke pluripotente stamcellen
Krivec, N., Spits, C. & Sermon, K.
1/11/20 → 31/10/24
Project: Fundamenteel
FWOSB35: FWO-SB-Beurs: het definiëren van de moleculaire basis voor levercel differentiatie deficiëntie in humane pluripotente stamcellen
Geens, M., Spits, C. & Sermon, K.
1/01/17 → 31/12/20
Project: Fundamenteel
IWT660: Effect van chromosomale afwijkingen op het differentiatiepotentieel en kwaadaardige kenmerken van menselijke embryonale stamcellen
Spits, C.
1/01/14 → 31/12/17
Project: Fundamenteel

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abstract = "Human pluripotent stem cells have a wide variety of potential applications, ranging from clinical translation to in vitro disease modeling. However, there is significant variation in the potential of individual cell lines to differentiate towards each of the three germ layers as a result of (epi)genetic background, culture conditions, and other factors. We describe here in detail a methodology to evaluate this bias using short directed differentiation towards neuroectoderm, mesendoderm, and definitive endoderm in combination with quantification by RT-qPCR and immunofluorescent stains.",

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AU - Markouli, Christina

AU - Spits, Claudia

PY - 2022

Y1 - 2022

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AB - Human pluripotent stem cells have a wide variety of potential applications, ranging from clinical translation to in vitro disease modeling. However, there is significant variation in the potential of individual cell lines to differentiate towards each of the three germ layers as a result of (epi)genetic background, culture conditions, and other factors. We describe here in detail a methodology to evaluate this bias using short directed differentiation towards neuroectoderm, mesendoderm, and definitive endoderm in combination with quantification by RT-qPCR and immunofluorescent stains.

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KW - Endoderm

KW - Germ Layers

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KW - Neural Plate

KW - Pluripotent Stem Cells

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JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

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Measuring Early Germ-Layer Specification Bias in Human Pluripotent Stem Cells

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Bibliografische nota

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Projecten

FWOTM1016: De impact van recurrente chromosomale afwijkingen op het groeivoordeel en de aanleg tot differentiatie van menselijke pluripotente stamcellen

FWOSB35: FWO-SB-Beurs: het definiëren van de moleculaire basis voor levercel differentiatie deficiëntie in humane pluripotente stamcellen

IWT660: Effect van chromosomale afwijkingen op het differentiatiepotentieel en kwaadaardige kenmerken van menselijke embryonale stamcellen

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