Method for preparing a lyophilized composition

The current invention relates to a method for preparing a composition of biomolecules, selected from peptides, small molecules, scaffold proteins, antibodies or antibody fragments, wherein said composition is lyophilized, characterized in that a vitamin C derivative is used as a lyophilization excipient, wherein the vitamin C derivative is an ascorbyl glucoside selected from the group of 2-O- -D- glucopyranosyl ascorbic acid, 2-O- -D-glucopyranosyl ascorbic acid, 5-O- -D- glucopyranosyl ascorbic acid, 6-O- -D-glucopyranosyl ascorbic acid, 3-O-glycosyl-L- ascorbic acid, 6-O-acyl-2-O- -D-glucopyranosyl ascorbic acid or a mixture thereof. The current invention also relates to a composition or a kit of lyophilized biomolecules, said biomolecules are chosen from the list of peptides, small molecules, scaffold proteins, antibodies or antibody fragments, said lyophilizate further comprises a vitamin C derivative, wherein said vitamin C derivative is chosen from the group of 2-O- -D-glucopyranosyl ascorbic acid, 2-O- -D-glucopyranosyl ascorbic acid, 5-O- -D-glucopyranosyl ascorbic acid, 6-O- -D-glucopyranosyl ascorbic acid, 3-O-glycosyl-L-ascorbic acid, 6-O-acyl-2-O- -D-glucopyranosyl ascorbic acid or a mixture thereof.