TY - JOUR
T1 - Microbial activity in herbivore dung affects nodulation and growth of Trifolium pratense
AU - Sun, Xingzhao
AU - M’Barek Bouanane, Amine
AU - Teixeira, Leonardo H.
AU - Sitters, Judith
AU - Olde Venterink, Harry
N1 - Funding Information:
We acknowledge permission for dung collection in the Kraansvlak (Nationaal Park Zuid Kennemerland) nature reserve of the PWN management and the rangers for their assistance, and Esther Rodrigues Gonzales for providing advisory. We thank Martin J Wassen for helping dung collection and guidance. We thank Dag Treer for sharing his experience with Bioinformatics, and Timothy Sierens for technical and administrative assistance. We thank CSC (China Scholarship Council) for the doctoral scholarship.
Publisher Copyright:
© The Author(s), under exclusive licence to Springer Nature Switzerland AG 2024.
PY - 2025/7
Y1 - 2025/7
N2 - Background and aims: Herbivore dung varies among species in terms of nutrients and microbial composition, but the effect of the microbial activity in the dung on plant growth and symbiotic association is largely unknown. This study aimed to investigate the influence of cow dung quantity and dung microbial activity on plant-microbe interactions (mycorrhiza, nodulation) and the growth of Trifolium pratense seedlings. Methods: A mesocosm experiment was conducted with gradients of unsterilized or sterilized cow dung applied to pots with T. pratense seedlings. Biomass, relative growth rate (RGR), root phosphomonoesterase (PME) activity, nodulation, and root mycorrhizal colonization of the seedling were measured after 7 weeks. Results: Growth of T. pratense increased with increasing dung supply, while sterilization marginally decreased growth by 15–20% (P = 0.068). Root nodulation increased with increasing dung supply but was significantly lower with sterilized dung. Both root PME activity and mycorrhizal colonization decreased with increasing dung supply but were unaffected by sterilization. Conclusions: The decreased growth and nodulation due to dung sterilization aligned with our predictions but could not be attributed to reduced dung decomposition and nutrient availability. Instead, the reduced microbial activity from sterilization likely negatively impacted bacterial N2-fixing activity and, consequently, plant growth. This suggests that herbivore dung’s effect on plant growth and interactions is more complex than previously anticipated, influenced not only by nutrient supply and stoichiometry but also by microbial composition and activity.
AB - Background and aims: Herbivore dung varies among species in terms of nutrients and microbial composition, but the effect of the microbial activity in the dung on plant growth and symbiotic association is largely unknown. This study aimed to investigate the influence of cow dung quantity and dung microbial activity on plant-microbe interactions (mycorrhiza, nodulation) and the growth of Trifolium pratense seedlings. Methods: A mesocosm experiment was conducted with gradients of unsterilized or sterilized cow dung applied to pots with T. pratense seedlings. Biomass, relative growth rate (RGR), root phosphomonoesterase (PME) activity, nodulation, and root mycorrhizal colonization of the seedling were measured after 7 weeks. Results: Growth of T. pratense increased with increasing dung supply, while sterilization marginally decreased growth by 15–20% (P = 0.068). Root nodulation increased with increasing dung supply but was significantly lower with sterilized dung. Both root PME activity and mycorrhizal colonization decreased with increasing dung supply but were unaffected by sterilization. Conclusions: The decreased growth and nodulation due to dung sterilization aligned with our predictions but could not be attributed to reduced dung decomposition and nutrient availability. Instead, the reduced microbial activity from sterilization likely negatively impacted bacterial N2-fixing activity and, consequently, plant growth. This suggests that herbivore dung’s effect on plant growth and interactions is more complex than previously anticipated, influenced not only by nutrient supply and stoichiometry but also by microbial composition and activity.
UR - http://www.scopus.com/inward/record.url?scp=85209754523&partnerID=8YFLogxK
U2 - 10.1007/s11104-024-07089-5
DO - 10.1007/s11104-024-07089-5
M3 - Article
SN - 0032-079X
VL - 512
SP - 429
EP - 440
JO - Plant and Soil
JF - Plant and Soil
IS - 1-2
ER -