Molecular cytogenetic characterization of four previously established and two newly established Ewing sarcoma cell lines

Károly Szuhai; Marije IJszenga; Hans J. Tanke; Carla Rosenberg; Pancras C.W. Hogendoorn

doi:10.1016/j.cancergencyto.2005.11.006

Molecular cytogenetic characterization of four previously established and two newly established Ewing sarcoma cell lines

Károly Szuhai
, Marije IJszenga
, Hans J. Tanke
, Carla Rosenberg
, Pancras C.W. Hogendoorn

Onderzoeksoutput: Article › peer review

32 Citaten (Scopus)

Samenvatting

Most Ewing family tumors are identified by the characteristic translocation t(11;22)(q24;q12), resulting in a fusion protein EWS/FLI1 that acts as an aberrant transcription factor. In a minority of cases, the EWS gene is fused to another member of the ETS gene (ERG, ETV1, E1AF, and FEV). Though the oncogenic transforming capability of the EWS/FLI1 protein is highly suggestive, the exact pathway behind remains to be elucidated. The availability of cell lines may help in the understanding of underlying cellular processes. In this study, we have established two new Ewing sarcoma cell lines and characterized them with molecular cytogenetic tools. This technology was also applied on four other previously published Ewing sarcoma cell lines. Our findings in relation to previous data on similar tumors are discussed.

Originele taal-2	English
Pagina's (van-tot)	173-179
Aantal pagina's	7
Tijdschrift	Cancer Genetics and Cytogenetics
Volume	166
Nummer van het tijdschrift	2
DOI's	https://doi.org/10.1016/j.cancergencyto.2005.11.006
Status	Published - 15 apr. 2006
Extern gepubliceerd	Ja

Bibliografische nota

Funding Information:
This project was supported by a grant from the Dutch Cancer Foundation (KWF2001-2526). We are grateful to Dr. S.A. Burchill (St. James' University Hospital, Leeds, UK) for kindly donating the TC-32, TTC-466 cell lines, Martine Peter (Laboratoire de Transfert, Institut Curie, Paris, France) for donating cell lines EW3 and EW7, and E. Kerkhof (Leiden University Medical Center, Leiden, the Netherlands) for performing the RT-PCR experiments.

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title = "Molecular cytogenetic characterization of four previously established and two newly established Ewing sarcoma cell lines",

abstract = "Most Ewing family tumors are identified by the characteristic translocation t(11;22)(q24;q12), resulting in a fusion protein EWS/FLI1 that acts as an aberrant transcription factor. In a minority of cases, the EWS gene is fused to another member of the ETS gene (ERG, ETV1, E1AF, and FEV). Though the oncogenic transforming capability of the EWS/FLI1 protein is highly suggestive, the exact pathway behind remains to be elucidated. The availability of cell lines may help in the understanding of underlying cellular processes. In this study, we have established two new Ewing sarcoma cell lines and characterized them with molecular cytogenetic tools. This technology was also applied on four other previously published Ewing sarcoma cell lines. Our findings in relation to previous data on similar tumors are discussed.",

author = "K{\'a}roly Szuhai and Marije IJszenga and Tanke, \{Hans J.\} and Carla Rosenberg and Hogendoorn, \{Pancras C.W.\}",

note = "Funding Information: This project was supported by a grant from the Dutch Cancer Foundation (KWF2001-2526). We are grateful to Dr. S.A. Burchill (St. James' University Hospital, Leeds, UK) for kindly donating the TC-32, TTC-466 cell lines, Martine Peter (Laboratoire de Transfert, Institut Curie, Paris, France) for donating cell lines EW3 and EW7, and E. Kerkhof (Leiden University Medical Center, Leiden, the Netherlands) for performing the RT-PCR experiments. ",

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AU - Szuhai, Károly

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AU - Tanke, Hans J.

AU - Rosenberg, Carla

AU - Hogendoorn, Pancras C.W.

N1 - Funding Information: This project was supported by a grant from the Dutch Cancer Foundation (KWF2001-2526). We are grateful to Dr. S.A. Burchill (St. James' University Hospital, Leeds, UK) for kindly donating the TC-32, TTC-466 cell lines, Martine Peter (Laboratoire de Transfert, Institut Curie, Paris, France) for donating cell lines EW3 and EW7, and E. Kerkhof (Leiden University Medical Center, Leiden, the Netherlands) for performing the RT-PCR experiments.

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N2 - Most Ewing family tumors are identified by the characteristic translocation t(11;22)(q24;q12), resulting in a fusion protein EWS/FLI1 that acts as an aberrant transcription factor. In a minority of cases, the EWS gene is fused to another member of the ETS gene (ERG, ETV1, E1AF, and FEV). Though the oncogenic transforming capability of the EWS/FLI1 protein is highly suggestive, the exact pathway behind remains to be elucidated. The availability of cell lines may help in the understanding of underlying cellular processes. In this study, we have established two new Ewing sarcoma cell lines and characterized them with molecular cytogenetic tools. This technology was also applied on four other previously published Ewing sarcoma cell lines. Our findings in relation to previous data on similar tumors are discussed.

AB - Most Ewing family tumors are identified by the characteristic translocation t(11;22)(q24;q12), resulting in a fusion protein EWS/FLI1 that acts as an aberrant transcription factor. In a minority of cases, the EWS gene is fused to another member of the ETS gene (ERG, ETV1, E1AF, and FEV). Though the oncogenic transforming capability of the EWS/FLI1 protein is highly suggestive, the exact pathway behind remains to be elucidated. The availability of cell lines may help in the understanding of underlying cellular processes. In this study, we have established two new Ewing sarcoma cell lines and characterized them with molecular cytogenetic tools. This technology was also applied on four other previously published Ewing sarcoma cell lines. Our findings in relation to previous data on similar tumors are discussed.

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Molecular cytogenetic characterization of four previously established and two newly established Ewing sarcoma cell lines

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