Mutagenesis by insertion of the drug resistance transposon Tn7 applied to the Ti plasmid of Agrobacterium tumefaciens

J. P. Hernalsteens, H. De Greve, M. Van Montagu, J. Schell

Onderzoeksoutput: Articlepeer review

30 Citaten (Scopus)

Samenvatting

Insertions of the linked trimethoprim and streptomycin resistance transposon Tn7 were isolated in a cointegrated plasmid formed between the Ti-plasmid of Agrobacterium tumefaciens strain B6S3 and the broad host range resistance plasmid RP4. By using the spontaneous dissociation of this cointegrate, we could demonstrate that these insertions had occurred into the Ti as well as into the RP4 part of the cointegrated plasmid. Among the insertions in the Ti part, mutants affected in different Ti plasmid-determined phenotypes, including non-oncogenic mutants, were detected. These mutants are useful for the physical mapping of these plasmid markers.

Originele taal-2English
Pagina's (van-tot)218-225
Aantal pagina's8
TijdschriftPlasmid
Volume1
Nummer van het tijdschrift2
DOI's
StatusPublished - 1 jan 1978

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