Novel method optimization for estrogenic activity assessment of steroid hormones in water using DGT and the ERE-CALUX bioassay.

Onderzoeksoutput: Meeting abstract (Book)

Samenvatting

Adverse effects of steroid estrogens and endocrine disrupting chemicals(EDCs) can occur at relatively low concentrations in the aquatic environment.Monitoring these compounds as encouraged by the European Water Framework Directives (WFD, 2013/39/EU) demands efficient, simple and sensitive techniques to detect and quantify these chemicals. Here, a novel sampling technique of diffusive gradients in thin films (DGT) combined with the chemically activated luciferase gene expression (ERE-CALUX) bioassay was developed for in situ pre-concentration and estrogenic effect measurement of steroids in the aquatic environment. In the study, the performance of this novel method was assessed,with 17β-estradiol (E2) as a model estrogen in a single compound solution, VM7Luc4E2 cells (formerly BG1Luc4E2) as ERE-CALUX bioassay cells and XAD 18 resin gel as a binding phase. The laboratory tests showed that DGT components and the experimental matrix do not influence the estrogenic activity of ERE-CALUX cells. XAD 18 showed sufficiently high capacity for the binding of E2. The measured effective diffusion coefficient ofE2 in agarose diffusive gel was 4.7 ×10-6cm2/s at 25 °C, which is slightly lower than the theoretical one (5.2 ×10-6cm2/s). The detection limit of this novel method  was significantly lower than the one obtained from active sampling combined with GC/MS and LC/MS analysis (0.1-7.0 ng/L).This method was independent of pH (5-8), ionic strength (0.001-0.1M) and dissolved organic matter concentrations (0-30 mg/L). This study demonstrates that DGT combined with ERE-CALUX is an effective tool for pre-concentrating steroid estrogens and possibly also in the monitoring of estrogenic activity in natural waters.Adverse effects of steroid estrogens and endocrine disrupting chemicals(EDCs) can occur at relatively low concentrations in the aquatic environment.Monitoring these compounds as encouraged by the European Water Framework Directives (WFD, 2013/39/EU) demands efficient, simple and sensitive techniques to detect and quantify these chemicals. Here, a novel sampling technique of diffusive gradients in thin films (DGT) combined with the chemically activated luciferase gene expression (ERE-CALUX) bioassay was developed for in situ pre-concentration and estrogenic effect measurement of steroids in the aquatic environment. In the study, the performance of this novel method was assessed,with 17β-estradiol (E2) as a model estrogen in a single compound solution, VM7Luc4E2 cells (formerly BG1Luc4E2) as ERE-CALUX bioassay cells and XAD 18 resin gel as a binding phase. The laboratory tests showed that DGT components and the experimental matrix do not influence the estrogenic activity of ERE-CALUX cells. XAD 18 showed sufficiently high capacity for the binding of E2. The measured effective diffusion coefficient ofE2 in agarose diffusive gel was 4.7 ×10-6cm2/s at 25 °C, which is slightly lower than the theoretical one (5.2 ×10-6cm2/s). The detection limit of this novel method  was significantly lower than the one obtained from active sampling combined with GC/MS and LC/MS analysis (0.1-7.0 ng/L).This method was independent of pH (5-8), ionic strength (0.001-0.1M) and dissolved organic matter concentrations (0-30 mg/L). This study demonstrates that DGT combined with ERE-CALUX is an effective tool for pre-concentrating steroid estrogens and possibly also in the monitoring of estrogenic activity in natural waters.Adverse effects of steroid estrogens and endocrine disrupting chemicals(EDCs) can occur at relatively low concentrations in the aquatic environment.Monitoring these compounds as encouraged by the European Water Framework Directives (WFD, 2013/39/EU) demands efficient, simple and sensitive techniques to detect and quantify these chemicals. Here, a novel sampling technique of diffusive gradients in thin films (DGT) combined with the chemically activated luciferase gene expression (ERE-CALUX) bioassay was developed for in situ pre-concentration and estrogenic effect measurement of steroids in the aquatic environment. In the study, the performance of this novel method was assessed,with 17β-estradiol (E2) as a model estrogen in a single compound solution, VM7Luc4E2 cells (formerly BG1Luc4E2) as ERE-CALUX bioassay cells and XAD 18 resin gel as a binding phase. The laboratory tests showed that DGT components and the experimental matrix do not influence the estrogenic activity of ERE-CALUX cells. XAD 18 showed sufficiently high capacity for the binding of E2. The measured effective diffusion coefficient ofE2 in agarose diffusive gel was 4.7 ×10-6cm2/s at 25 °C, which is slightly lower than the theoretical one (5.2 ×10-6cm2/s). The detection limit of this novel method  was significantly lower than the one obtained from active sampling combined with GC/MS and LC/MS analysis (0.1-7.0 ng/L).This method was independent of pH (5-8), ionic strength (0.001-0.1M) and dissolved organic matter concentrations (0-30 mg/L). This study demonstrates that DGT combined with ERE-CALUX is an effective tool for pre-concentrating steroid estrogens and possibly also in the monitoring of estrogenic activity in natural waters.Adverse effects of steroid estrogens and endocrine disrupting chemicals(EDCs) can occur at relatively low concentrations in the aquatic environment.Monitoring these compounds as encouraged by the European Water Framework Directives (WFD, 2013/39/EU) demands efficient, simple and sensitive techniques to detect and quantify these chemicals. Here, a novel sampling technique of diffusive gradients in thin films (DGT) combined with the chemically activated luciferase gene expression (ERE-CALUX) bioassay was developed for in situ pre-concentration and estrogenic effect measurement of steroids in the aquatic environment. In the study, the performance of this novel method was assessed,with 17β-estradiol (E2) as a model estrogen in a single compound solution, VM7Luc4E2 cells (formerly BG1Luc4E2) as ERE-CALUX bioassay cells and XAD 18 resin gel as a binding phase. The laboratory tests showed that DGT components and the experimental matrix do not influence the estrogenic activity of ERE-CALUX cells. XAD 18 showed sufficiently high capacity for the binding of E2. The measured effective diffusion coefficient ofE2 in agarose diffusive gel was 4.7 ×10-6cm2/s at 25 °C, which is slightly lower than the theoretical one (5.2 ×10-6cm2/s). The detection limit of this novel method  was significantly lower than the one obtained from active sampling combined with GC/MS and LC/MS analysis (0.1-7.0 ng/L).This method was independent of pH (5-8), ionic strength (0.001-0.1M) and dissolved organic matter concentrations (0-30 mg/L). This study demonstrates that DGT combined with ERE-CALUX is an effective tool for pre-concentrating steroid estrogens and possibly also in the monitoring of estrogenic activity in natural waters.

Originele taal-2English
TitelSETAC Brussels Abstract Book
SubtitelEnvironmental quality through transdisciplinary collaboration
Pagina's308
Aantal pagina's1
ISBN van elektronische versie2310-3043
StatusPublished - 11 mei 2017
EvenementSETAC Europe 27th Annual Meeting: Environmental Quality through Transdisciplinary Collaboration - Square Meeting Center Brussels, Brussels, Belgium
Duur: 7 mei 201711 mei 2017
https://brussels.setac.org/
https://brussels.setac.org/
https://brussels.setac.org/
https://brussels.setac.org/

Conference

ConferenceSETAC Europe 27th Annual Meeting
Land/RegioBelgium
StadBrussels
Periode7/05/1711/05/17
Internet adres

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