Omentum is better site than kidney capsule for growth, differentiation, and vascularization of immature porcine β-cell implants in immunodeficient rats.

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Samenvatting

Background. Rapid revascularization of islet cell implants is important for engraftment and subsequent survival and function. Development of an adequate vascular network is expected to allow adaptive growth of the A-cell mass. The present study compares omentum and kidney capsule as sites for growth and differentiation of immature A-cell grafts.

Methods. Perinatal porcine islet cell grafts were implanted in omentum or under kidney capsule of nondiabetic nude rats. Implants were compared over 10 weeks for their respective growth, cellular composition, number and size of A cells, their proliferative activity, and implant blood vessel density.

Results. In both sites, the A-cell volume increased fourfold between weeks 1 and 10 reflecting a rise in A-cell number. In the omental implants, however, the cellular insulin reserves and the percent of proliferating cells were twofold higher than in kidney implants. In parallel, the blood vessel density in omental implants increased twofold, reaching a density comparable with islets in adult pig pancreas. A positive correlation was found between the percent bromodeoxyuridine-positive A cells and the vessel density.

Conclusions. Growth of the A-cell volume proceeds similarly in the omentum and under the kidney capsule. However, the omentum leads to higher insulin reserves and an increased pool of proliferating cells, which might
be related to a more extended vascular network. Our observations support the omentum as an alternative site for
immature porcine islet cells, with beneficial effects on proliferation and implant revascularization.
Originele taal-2English
Pagina's (van-tot)1026-1033
Aantal pagina's8
TijdschriftTransplantation
Volume96
StatusPublished - 2013

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