Prototyping of thermoplastic microfluidic chips and their application in high-performance liquid chromatography separations of small molecules

Sam Wouters, Jelle De Vos, José Luís Das Dores Sousa, Bert Wouters, Gert Desmet, Sebastiaan Eeltink

Onderzoeksoutput: Articlepeer review

28 Citaten (Scopus)

Samenvatting

The present paper discusses practical aspects of prototyping of microfluidic chips using cyclic olefin copolymer as substrate and the application in high-performance liquid chromatography. The developed chips feature a 60 mm long straight separation channel with circular cross section (500 mu m i.d.) that was created using a micromilling robot. To irreversibly seal the top and bottom chip substrates, a solvent vapor-assisted bonding approach was optimized, allowing to approximate the ideal circular channel geometry. Four different approaches to establish the micro-to-macro interface were pursued. The average burst pressure of the microfluidic chips in combination with an encasing holder was established at 38 MPa and the maximum burst pressure was 47 MPa, which is believed to be the highest ever report for these polymer-based microfluidic chips. Porous polymer monolithic frits were synthesized in-situ via UV-initiated polymerization and their locations were spatially controlled by the application of a photomask. Next, high-pressure slurry packing was performed to introduce 3 mu m silica reversed-phase particles as the stationary phase in the separation channel. Finally, the application of the chip technology is demonstrated for the separation of alkyl phenones in gradient mode yielding baseline peak widths of 6 s by applying a steep gradient of 1.8 min at a flow rate of 10 mu L/min. (C) 2017 Elsevier B.V. All rights reserved.
Originele taal-2English
Pagina's (van-tot)224-233
Aantal pagina's9
TijdschriftJournal of Chromatography A
Volume1523
DOI's
StatusPublished - 10 nov 2017

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