Projecten per jaar
Samenvatting
In this study, we deep-sequenced the mtDNA of human embryonic and induced pluripotent stem cells (hESCs and hiPSCs) and their source cells and found that the majority of variants pre-existed in the cells used to establish the lines. Early-passage hESCs carried few and low-load heteroplasmic variants, similar to those identified in oocytes and inner cell masses. The number and heteroplasmic loads of these variants increased with prolonged cell culture. The study of 120 individual cells of early- and late-passage hESCs revealed a significant diversity in mtDNA heteroplasmic variants at the single-cell level and that the variants that increase during time in culture are always passenger to the appearance of chromosomal abnormalities. We found that early-passage hiPSCs carry much higher loads of mtDNA variants than hESCs, which single-fibroblast sequencing proved pre-existed in the source cells. Finally, we show that these variants are stably transmitted during short-term differentiation.
Originele taal-2 | English |
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Pagina's (van-tot) | 102-114 |
Aantal pagina's | 13 |
Tijdschrift | Stem Cell Reports |
Volume | 11 |
Nummer van het tijdschrift | 1 |
DOI's | |
Status | Published - 10 jul 2018 |
Vingerafdruk
Duik in de onderzoeksthema's van 'Random Mutagenesis, Clonal Events, and Embryonic or Somatic Origin Determine the mtDNA Variant Type and Load in Human Pluripotent Stem Cells'. Samen vormen ze een unieke vingerafdruk.Projecten
- 2 Afgelopen
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FWOKN282: De studie van mutaties in het mitochondriaal genoom van humane pluripotente stamcellen door middel van massive parallel sequencing
1/01/16 → 31/12/16
Project: Fundamenteel
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OZRMETH3: Research at the interface between human genetics and reproduction.
Sermon, K., Seneca, S., Bonduelle, M., De Rycke, M., Devroey, P., Goossens, E., Lissens, W., Spits, C., Stouffs, K., Tournaye, H., Dee, K., Vandermaelen, D., Van Haute, L., Van Saen, D., Van Den Abbeel, E., Liebaers, I., De Meirleir, L., Nekkebroeck, J. & Van De Velde, H.
1/01/09 → 31/12/24
Project: Fundamenteel