Hemoglobinopathies are the most common genetic disorders caused by a mutation in the genes encoding for one of the globin chains and leading to structural (hemoglobin [Hb] variants) or quantitative defects (thalassemias) in hemoglobin. Early diagnosis and characterization of hemoglobinopathies are essential to avoid severe hematological consequences in the offspring of healthy carriers of a mutation. Despite being extensively studied, hemoglobinopathies continue to provide a diagnostic challenge. Sickle-cell hemoglobin (HbS) is the most common and clinically significant hemoglobin variant among all Hb variants. To overcome the challenge of diagnosing Hb variants, we propose the use of Surface-Enhanced Raman Spectroscopy (SERS). SERS is a powerful label-free tool for providing fingerprint structural information of analyses. It can rapidly generate the spectral signature of samples. This study investigates the structural differences between HbS and normal Hb using gold nanopillar SERS substrates with a leaning effect. The SERS spectra of Hb variants showed subtle spectral differences between HbS and normal Hb located in the valine (975cm-1) and glutamic acid (1547cm-1) band, reflecting the amino acid substitution in the HbS β-globin chain. We also automated the identification of HbS and normal Hb with principal component analysis (PCA) combined with support vector machine (SVM) and linear discriminant analysis (LDA) classifiers, leading to an accuracy of 98% and 96%, respectively. This study demonstrated that SERS can provide a fast, highly sensitive, noninvasive, and accurate detection module for the diagnosis of Sickle-cell disease and potentially other hemoglobinopathies.

Originele taal-2English
TitelEnhanced Spectroscopies and Nanoimaging 2023
RedacteurenPrabhat Verma, Yung Doug Suh
Aantal pagina's5
ISBN van elektronische versie9781510665224
StatusPublished - 2023
EvenementConference on Enhanced Spectroscopies and Nanoimaging - San Diego, United States
Duur: 20 aug 202323 aug 2023

Publicatie series

NaamProceedings of SPIE - The International Society for Optical Engineering
ISSN van geprinte versie0277-786X
ISSN van elektronische versie1996-756X


ConferenceConference on Enhanced Spectroscopies and Nanoimaging
Land/RegioUnited States
StadSan Diego

Bibliografische nota

Publisher Copyright:
© 2023 SPIE.


Duik in de onderzoeksthema's van 'SERS biosensing of Sickle cell hemoglobin from Normal hemoglobin'. Samen vormen ze een unieke vingerafdruk.

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