BACKGROUND: Cryopreservation of testicular tissue is an option for fertility preservation in reproductive stem cell loss disorders. The present study compared three methods for cryopreservation of human testicular tissue. MATERIALS AND METHODS: Testicular tissue, donated during vasectomy reversal, was used for comparison of controlled slow freezing (CSF), uncontrolled slow freezing (USF) and vitrification. In all methods, dimethyl sulphoxide (DMSO) was used as a cryoprotectant. Histological, immunohistochemical and ultrastructural analyses were performed to assess the tissue after cryopreservation. RESULTS: USF preserved the morphological characteristics of seminiferous tubules well. Tubules were more altered after CSF and vitrification. A good recovery of spermatogonia was observed after USF and vitrification while CSF resulted in a significant loss of spermatogonia. Sertoli cell recovery was comparable between all cryopreservation groups. As revealed by electron microscopy, the cell ultrastructure was best preserved after USF and vitrification. When CSF was used, more deleterious cryoinjury to cellular components was observed. CONCLUSION: USF and vitrification are easy and inexpensive alternatives for cryopreservation of human testicular tissue. Supplementary research is required to investigate the effect on tissue functionality and to confirm this study's findings using prepubertal tissue.
|Status||Published - 28 sep 2011|
|Evenement||Unknown - |
Duur: 28 sep 2011 → …
|Periode||28/09/11 → …|