Stratifying fascin and cortactin function in invadopodium formation using inhibitory nanobodies and targeted subcellular delocalization

Isabel Van Audenhove, Ciska Boucherie, L. Pieters, Olivier Zwaenepoel, Berlinda Van Loo, E. Martens, Charlotte Verbrugge, Gholamreza Hassanzadeh Ghassabeh, Joel Vandekerckhove, Maria Cornelissen, Ariane De Ganck, Jan Gettemans

Onderzoeksoutput: Articlepeer review

48 Citaten (Scopus)

Samenvatting

Invadopodia are actin-rich protrusions arising through the orchestrated regulation of precursor assembly, stabilization, and maturation, endowing cancer cells with invasive properties. Using nanobodies (antigen-binding domains of Camelid heavy-chain antibodies) as perturbators of intracellular functions and/or protein domains at the level of the endogenous protein, we examined the specific contribution of fascin and cortactin during invadopodium formation in MDA-MB-231 breast and PC-3 prostate cancer cells. A nanobody (K(d)~35 nM, 1:1 stoichiometry) that disrupts fascin F-actin bundling emphasizes the importance of stable actin bundles in invadopodium array organization and turnover, matrix degradation, and cancer cell invasion. Cortactin-SH3 dependent WIP recruitment toward the plasma membrane was specifically inhibited by a cortactin nanobody (K(d)~75 nM, 1:1 stoichiometry). This functional domain is shown to be important for formation of properly organized invadopodia, MMP-9 secretion, matrix degradation, and cancer cell invasion. Notably, using a subcellular delocalization strategy to trigger protein loss of function, we uncovered a fascin-bundling-independent role in MMP-9 secretion. Hence, we demonstrate that nanobodies enable high resolution protein function mapping in cells.
Originele taal-2English
Pagina's (van-tot)1805-1818
TijdschriftThe FASEB Journal
Volume28
Nummer van het tijdschrift4
StatusPublished - apr 2014

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