Structural Characterization of Monomeric/Dimeric State of p59(fyn) SH2 Domain

Radu Huculeci, Fabien Kieken, Abel Garcia-Pino, Lieven Buts, Nico van Nuland, Tom Lenaerts

Onderzoeksoutput: Articlepeer review

1 Citaat (Scopus)

Samenvatting

Src homology 2 (SH2) domains are key modulators in various signaling pathways allowing the recognition of phosphotyrosine sites of different proteins. Despite the fact that SH2 domains acquire their biological functions in a monomeric state, a multitude of reports have shown their tendency to dimerize. Here, we provide a technical description on how to isolate and characterize by gel filtration, circular dichroism (CD), and nuclear magnetic resonance (NMR) each conformational state of p59(fyn) SH2 domain.

Originele taal-2English
Pagina's (van-tot)257-267
Aantal pagina's11
TijdschriftMethods in Molecular Biology
Volume1555
DOI's
StatusPublished - 15 jan 2017

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