Structure-function relationship of new peptides activating human Nav1.1

Ludivine Lopez, Stephan De Waard, Hervé Meudal, Cécile Caumes, Kuldip Khakh, Steve Peigneur, Barbara Oliveira-Mendes, Sophia Lin, Jolien De Waele, Jérôme Montnach, Sandrine Cestèle, Agnès Tessier, J P Johnson, Massimo Mantegazza, Jan Tytgat, Charles Cohen, Rémy Béroud, Frank Bosmans, Céline Landon, Michel De Waard

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3 Citaten (Scopus)

Samenvatting

Nav1.1 is an important pharmacological target as this voltage-gated sodium channel is involved in neurological and cardiac syndromes. Channel activators are actively sought to try to compensate for haploinsufficiency in several of these pathologies. Herein we used a natural source of new peptide compounds active on ion channels and screened for drugs capable to inhibit channel inactivation as a way to compensate for decreased channel function. We discovered that JzTx-34 is highly active on Nav1.1 and subsequently performed a full structure-activity relationship investigation to identify its pharmacophore. These experiments will help interpret the mechanism of action of this and formerly identified peptides as well as the future identification of new peptides. We also reveal structural determinants that make natural ICK peptides active against Nav1.1 challenging to synthesize. Altogether, the knowledge gained by this study will help facilitate the discovery and development of new compounds active on this critical ion channel target.

Originele taal-2English
Artikelnummer115173
Aantal pagina's15
TijdschriftBiomedicine & Pharmacotherapy
Volume165
DOI's
StatusPublished - sep 2023

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Copyright © 2023 The Authors. Published by Elsevier Masson SAS.. All rights reserved.

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