T-DNA organization in tumor cultures and transgenic plants of the monocotyledon Asparagus officinalis

Benny Bytebier, Francine Deboeck, Henri De Greve, Marc Van Montagu, Jean-Pierre Hernalsteens

Onderzoeksoutput: Articlepeer review

Samenvatting

Asparagus officinalis was the first monocotyledonous plant from which hormone-independent and opine-producing crown gall tissue could be isolated. We confirm by DNA hybridization that tumor lines obtained after infection of this plant by Agrobacterium strains harboring wild-type nopaline and octopine tumor-inducing (Ti) plasmids are stably transformed and contain transferred DNA (T-DNA) segments identical to the T-DNA found in dicotyledonous plants. We have also infected Asparagus with a nononcogenic T-DNA vector that carries a chimeric aminoglycoside phosphotransferase [NOS-APH(3')II] gene and selected transformed tissues on kanamycin-containing medium. The transformed status of these tissues was then confirmed by DNA hybridization. From these calli we regenerated kanamycin-resistant shoots that were subsequently rooted. Thus we report the isolation of transgenic monocotyledonous plants engineered via the Agrobacterium vector system.
Originele taal-2English
Pagina's (van-tot)5345-5349
Aantal pagina's5
TijdschriftProc Natl Acad Sci USA
Volume84
StatusPublished - aug 1987

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