Background: Intrapulmonary percussive ventilation (IPV) is an airway clearance technique used at any age to facilitate the removal of secretions from the respiratory tract. The effect of IPV on gastroesophageal reflux(GER) is not well documented. This study aimed to determine the influence of IPV on GER in infants. Methods: During a 24-hour multichannel intraluminal impedance-pH monitoring (MII-pH), infants in the intervention group received a20 minutes session of IPV in upright position, 2 hours postprandial. The control group received no intervention and was placed in the same position for 20 minutes, 2 hours postprandial. The number of reflux episodes (REs) during IPV as compared to the number of REs in the control group during the 20 minutes period. Results: Fifty infants were included in each group;21 infants in the IPV group and 14 infants in the control group were diagnosed with abnormal MII-pH (P =.142). During IPV, the number of REs was significantly lower than in the control group (P <.001). Also in the subgroups with abnormal or normal MII-pH, a significantly lower number of REs during IPV was found (P =.011, P =.011 respectively). Conclusion: IPV decreases the number of REs in infants independent of the results of MII-pH.