TY - JOUR
T1 - Transcriptomic profiling of different responder types in adults after a Priorix® vaccination
AU - Bartholomeus, Esther
AU - De Neuter, Nicolas
AU - Suls, Arvid
AU - Elias, George
AU - van der Heijden, Sanne
AU - Keersmaekers, Nina
AU - Jansens, Hilde
AU - Van Tendeloo, Viggo
AU - Beutels, Philippe
AU - Laukens, Kris
AU - Ogunjimi, Benson
AU - Mortier, Geert
AU - Meysman, Pieter
AU - Van Damme, Pierre
N1 - Copyright © 2020 Elsevier Ltd. All rights reserved.
PY - 2020/4/3
Y1 - 2020/4/3
N2 - Thanks to the recommendation of a combined Measles/Mumps/Rubella (MMR) vaccine, like Priorix®, these childhood diseases are less common now. This is beneficial to limit the spread of these diseases and work towards their elimination. However, the measles, mumps and rubella antibody titers show a large variability in short- and long-term immunity. The recent outbreaks worldwide of measles and mumps and previous studies, which mostly focused on only one of the three virus responses, illustrate that there is a clear need for better understanding the immune responses after vaccination. Our healthy cohort was already primed with the MMR antigens in their childhood. In this study, the adult volunteers received one Priorix® vaccine dose at day 0. First, we defined 4 different groups of responders, based on their antibody titers' evolution over 4 time points (Day 0, 21, 150 and 365). This showed a high variability within and between individuals. Second, we determined transcriptome profiles using 3'mRNA sequencing at day 0, 3 and 7. Using two analytical approaches, "one response group per time point" and "a time comparison per response group", we correlated the short-term gene expression profiles to the different response groups. In general, the list of differentially expressed genes is limited, however, most of them are clearly immune-related and upregulated at day 3 and 7, compared to the baseline day 0. Depending on the specific response group there are overlapping signatures for two of the three viruses. Antibody titers and transcriptomics data showed that an additional Priorix vaccination does not facilitate an equal immune response against the 3 viruses or among different vaccine recipients.
AB - Thanks to the recommendation of a combined Measles/Mumps/Rubella (MMR) vaccine, like Priorix®, these childhood diseases are less common now. This is beneficial to limit the spread of these diseases and work towards their elimination. However, the measles, mumps and rubella antibody titers show a large variability in short- and long-term immunity. The recent outbreaks worldwide of measles and mumps and previous studies, which mostly focused on only one of the three virus responses, illustrate that there is a clear need for better understanding the immune responses after vaccination. Our healthy cohort was already primed with the MMR antigens in their childhood. In this study, the adult volunteers received one Priorix® vaccine dose at day 0. First, we defined 4 different groups of responders, based on their antibody titers' evolution over 4 time points (Day 0, 21, 150 and 365). This showed a high variability within and between individuals. Second, we determined transcriptome profiles using 3'mRNA sequencing at day 0, 3 and 7. Using two analytical approaches, "one response group per time point" and "a time comparison per response group", we correlated the short-term gene expression profiles to the different response groups. In general, the list of differentially expressed genes is limited, however, most of them are clearly immune-related and upregulated at day 3 and 7, compared to the baseline day 0. Depending on the specific response group there are overlapping signatures for two of the three viruses. Antibody titers and transcriptomics data showed that an additional Priorix vaccination does not facilitate an equal immune response against the 3 viruses or among different vaccine recipients.
UR - http://www.scopus.com/inward/record.url?scp=85081214753&partnerID=8YFLogxK
U2 - 10.1016/j.vaccine.2020.03.004
DO - 10.1016/j.vaccine.2020.03.004
M3 - Article
C2 - 32165045
VL - 38
SP - 3218
EP - 3226
JO - Vaccine
JF - Vaccine
SN - 0264-410X
IS - 16
M1 - 32165045
ER -