Transfer of Ti plasmids between Agrobacterium strains by mobilisation with the conjugative plasmid RP4

N. Van Larebeke, Ch Genetello, J. P. Hernalsteens, A. De Picker, I. Zaenen, E. Messens, M. Van Montagu, J. Schell

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88 Citaten (Scopus)

Samenvatting

The P type conjugative plasmid RP4 has been shown to be able to promote the transfer of the Agrobacterium Ti-plasmid. The results provide additional evidence that agrocin 84 sensitivity, exclusion of phage AP1, ability to catabolize the guanidine derivatives octopine and nopaline and tumor inducing ability, are Ti-plasmid determined properties. Furthermore, the results strongly support the notion that at least part of the Ti-plasmid is transferred from the bacterium to the target plant cells, since it was demonstrated that Ti-plasmid linked genes specify the synthesis of octopine or nopaline by crown-gall tumor cells.

Originele taal-2English
Pagina's (van-tot)119-124
Aantal pagina's6
TijdschriftMGG Molecular & General Genetics
Volume152
Nummer van het tijdschrift2
DOI's
StatusPublished - 1 jan 1977

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