Plant IDPs Arabidopsis thaliana ERD10 and ERD14: in planta characterization

Samenvatting

Early responsive dehydration 10 and 14 proteins (ERD10 and ERD14) belongs to group 2 – Late embryogenesis abundant proteins (LEA proteins). They were also called the dehydrins proteins and were proved to be intrinsically disordered. Some studies indicated that the dehydrins of several plants species played a role in protecting plants subjected to stress conditions such as: drought, salt and cold stresses. In the framework of this thesis: ERD10 and ERD14 of Arabidopsis thaliana were used to unreal the role of these dehydrins in stress tolerance of Arabidopsis. Arabidopsis plants with different expression levels of ERD10 and ERD14 were treated with several concentrations of mannitol (drought stress) and NaCl (salt stress). Under stress conditions, the overexpression plants expressed ERD10 and ERD14 much higher than wild type and knockout lines under the same conditions. The overexpression lines also showed better growth than the others subjected to stresses. The results presented significant effects of ERD10 and ERD14 on plant development including plant growth and flowering time of plants under stress conditions. Especially, under two equiosmolar conditions mannitol and NaCl, ERD10 and ERD14 had significantly difference in their response to long period of stress treatments. The localization of ERD10 on the membrane of plant cells subjected to salt and cold stresses was also determined.

Datum prijs	13 sep. 2013
Originele taal	English
Prijsuitreikende instantie	KU Leuven Vrije Universiteit Brussel Universiteit Antwerpen and Vrije Universiteit Brussel
Begeleider	Geert Angenon (Promotor) & Peter Tompa (Promotor)